Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody

Melan-A/MART-1 antibody

Boster Bio Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody (Catalog # M02033). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.

Product Info Summary

SKU: M02033
Size: 100ug/vial
Reactive Species: Human, Horse
Host: Mouse
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody

View all Melan-A/MART-1 Antibodies

SKU/Catalog Number

M02033

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody (Catalog # M02033). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02033)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM342

Isotype

IgG2b, lambda

Immunogen

Recombinant hMART-1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02033 is reactive to MLANA in Human, Horse

Applications

M02033 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

13.157kDa

Background of Melan-A/MART-1

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MLANA (Source: Uniprot.org, NCBI)

Gene Name

MLANA

Full Name

Melanoma antigen recognized by T-cells 1

Weight

13.157kDa

Alternative Names

Antigen LB39-AA; Antigen SK29-AA; Mart 1 Melan A; MART-1; MART1MART-1; MelanA; Melan-A; melanoma antigen recognized by T-cells 1; MLANA; Protein Melan-A MLANA MART-1, MART1 melan-A melanoma antigen recognized by T-cells 1|antigen LB39-AA|antigen SK29-AA|epididymis secretory sperm binding protein|protein Melan-A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MLANA, check out the MLANA Infographic

MLANA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MLANA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody

Question

I see that the anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody M02033 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-19

Question

Is this M02033 anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody reactive to the isotypes of MLANA?

G. Banerjee

Verified customer

Asked: 2019-02-11

Answer

The immunogen of M02033 anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody is Recombinant hMART-1 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-11

Question

I was wanting to use to test anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody M02033 on human material anatomical entity for research purposes, then I may be interested in using anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody M02033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-18

Answer

The products we sell, including anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody M02033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-18

Question

I was wanting to use your anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody for IHC for human material anatomical entity on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human material anatomical entity identification?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

It shows on the product datasheet, M02033 anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal antibody has been tested for IHC on equine, human tissues. We have an innovator award program that if you test this antibody and show it works in human material anatomical entity in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-15

Order DetailsPrice
M02033-100ug

100 μg

$468

Free Secondary Antibody

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M02033
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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