Anti-MCG10 PCBP4 Antibody

Product Name

Anti-MCG10 PCBP4 Antibody

View all PCBP4 Antibodies

SKU/Catalog Number

A11870

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MCG10 PCBP4 Antibody (Catalog # A11870). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

MCG10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MCG10 PCBP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11870)

Host

Rabbit

Contents

MCG10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MCG10 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10. The immunogen is located within the last 50 amino acids of MCG10.

Reactive Species

A11870 is reactive to PCBP4 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

41.5 kDa

Background of PCBP4

MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11870 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PCBP4

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