Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Mouse;Rat.
|Applications||IHC, ICC, WB|
|Product Name||Anti-MCM2 Antibody
See all Mcm2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MCM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. MCM2 information: Molecular Weight: 102078 MW; Subcellular Localization: Nucleus .|
|Cite This Product||Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1711)|
|Specificity||Anti-MCM2 Antibody (PA1711) reacts with Mouse, Rat Mcm2, in native form and recombinant. Superfamily members of Mcm2 are not reactive to PA1711.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2(884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid.|
Our Boster Quality Guarantee for Anti-MCM2 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunocytochemistry , 0.5-1μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-MCM2 Antibody (PA1711).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Images And Assay Conditions
Anti-MCM2 antibody, PA1711, IHC(P)
IHC(P): Rat Intestinal Lymphocyte Tissue
Anti-MCM2 antibody, PA1711, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-MCM2 antibody, PA1711, Western blotting
All lanes: Anti MCM2 (PA1711) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Intestine Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: Mouse Ovary Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Predicted bind size: 102KD
Observed bind size: 102KD
Anti-MCM2 antibody, PA1711, ICC
ICC: HEPA Cell
Anti-MCM2 antibody, PA1711, ICC
ICC: NIH3T3 Cell
Protein Target Info (Source: Uniprot.org)
|Protein Name||DNA replication licensing factor MCM2|
|Alternative Names||DNA replication licensing factor MCM2;188.8.131.52;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;Mcm2;Bm28, Cdcl1, Kiaa0030, Mcmd2;|
|Subcellular Localization||Nucleus .|
|Molecular Weight||102078 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.|
|Research Areas||Mouse, Rat
*You can search these to find other products in these research areas.
|Background||MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.|
Other Recommended Resources
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Guaranteed product quality
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to mcm2 antibody, ccnl1 antibody