Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Mouse;Rat.
Anti-MCM2 Antibody Info At A Glance
|Applications:||IHC, ICC, WB|
CD1a is a transmembrane glycoproteins, which is structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1a mediates the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined.
|Product Name||Anti-MCM2 Antibody
See all Mcm2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MCM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. MCM2 information: Molecular Weight: 102078 MW; Subcellular Localization: Nucleus .|
|Cite This Product||Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1711)|
|Similar Products From
|Anti-MCM2 Antibody may replace the following items: sc 9839|sc 10771|sc 373702|sc 28551|sc 56322|sc 73572|sc 56321|sc 6680.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2(884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||DNA replication licensing factor MCM2|
|Subcellular Localization||Nucleus .|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-MCM2 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Rat Intestinal Lymphocyte Tissue
IHC(P): Mouse Intestine Tissue
All lanes: Anti MCM2 (PA1711) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Intestine Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: Mouse Ovary Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Predicted bind size: 102KD
Observed bind size: 102KD
ICC: HEPA Cell
ICC: NIH3T3 Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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