Anti-MCM2 Antibody

MCM2 antibody

Boster Bio Anti-MCM2 Antibody catalog # PA1650. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: PA1650
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-MCM2 Antibody

View all MCM2 Antibodies

SKU/Catalog Number

PA1650

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCM2 Antibody catalog # PA1650. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1650)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1650 is reactive to MCM2 in Human, Mouse

Applications

PA1650 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

125 kDa

Calculated molecular weight

101.896kDa

Background of MCM2

MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MCM2 (Source: Uniprot.org, NCBI)

Gene Name

MCM2

Full Name

DNA replication licensing factor MCM2

Weight

101.896kDa

Superfamily

MCM family

Alternative Names

BM28; BM28MGC10606; CCNL 1; CCNL1; cdc19; CDCL1; CDCL1mitotin; D3S3194; EC 3.6.4.12; KIAA0030MITOTIN; MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae); MCM2; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); Mitotin MCM2 BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 minichromosome maintenance complex component 2 DNA replication licensing factor MCM2|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MCM2, check out the MCM2 Infographic

MCM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MCM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-MCM2 Antibody

Question

Is a blocking peptide available for product anti-MCM2 antibody (PA1650)?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

We do provide the blocking peptide for product anti-MCM2 antibody (PA1650). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-24

Question

We have observed staining in mouse placenta uterus. Any tips? Is anti-MCM2 antibody supposed to stain placenta uterus positively?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

Based on literature placenta uterus does express MCM2. Based on Uniprot.org, MCM2 is expressed in oocyte, colon carcinoma, bone marrow, brain, lung, lymph, muscle, placenta uterus, cervix carcinoma, prostate cancer, embryonic kidney, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-03-05

Question

Can you help my question with product PA1650, anti-MCM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1650 anti-MCM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-23

Question

I am interested in to test anti-MCM2 antibody PA1650 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-MCM2 antibody PA1650 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

The products we sell, including anti-MCM2 antibody PA1650, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-12

Question

Is this PA1650 anti-MCM2 antibody reactive to the isotypes of MCM2?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The immunogen of PA1650 anti-MCM2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2(889-904aa NKFSHDLKRKMILQQF), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-29

Question

I see that the anti-MCM2 antibody PA1650 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-20

Question

We purchased anti-MCM2 antibody for WB on leukemic t-cell in the past. I am using human, and We intend to use the antibody for IHC next. I was wanting to use examining leukemic t-cell as well as embryonic kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

I took a look at the website and datasheets of our anti-MCM2 antibody and I see that PA1650 has been tested on human in both WB and IHC. Thus PA1650 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-01-31

Question

Does PA1650 anti-MCM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

It shows on the product datasheet, PA1650 anti-MCM2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-09

Question

Would anti-MCM2 antibody PA1650 work for IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

According to the expression profile of cervix carcinoma, MCM2 is highly expressed in cervix carcinoma. So, it is likely that anti-MCM2 antibody PA1650 will work for IHC with cervix carcinoma.

Boster Scientific Support

Answered: 2018-01-01

Question

I was wanting to use your anti-MCM2 antibody for IHC for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-09-08

Answer

It shows on the product datasheet, PA1650 anti-MCM2 antibody has been tested for IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-08

Question

We need using your anti-MCM2 antibody for g1/s transition of mitotic cell cycle studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

H. Miller

Verified customer

Asked: 2017-01-10

Answer

We appreciate your inquiry. This PA1650 anti-MCM2 antibody is tested on sw620 cell lysate, panc cell lysate, jurkat cell lysate, hela cell lysate, lung cancer tissue, intestinal cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-01-10

Question

Is there a BSA free version of anti-MCM2 antibody PA1650 available?

M. Krishna

Verified customer

Asked: 2016-01-14

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MCM2 antibody PA1650 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-01-14

Question

My boss were well pleased with the WB result of your anti-MCM2 antibody. However we have observed positive staining in prostate cancer nucleus. using this antibody. Is that expected? Could you tell me where is MCM2 supposed to be expressed?

D. Jackson

Verified customer

Asked: 2015-01-05

Answer

From what I have seen in literature, prostate cancer does express MCM2. Generally MCM2 expresses in nucleus. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2015-01-05

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-MCM2 antibody PA1650. Let me know if you need anything else.

K. Parker

Verified customer

Asked: 2015-01-02

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-01-02

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-MCM2 antibody PA1650. Please let me know if you require anything else.

K. Yang

Verified customer

Asked: 2014-09-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-09-22

Question

We are currently using anti-MCM2 antibody PA1650 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?

N. Yang

Verified customer

Asked: 2014-03-03

Answer

The anti-MCM2 antibody (PA1650) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-03

Order DetailsPrice
PA1650

100μg

$370
PA1650-10ug

10μg sample (liquid)

$99
PA1650-Biotin

100 μg Biotin conjugated

$570
PA1650-Cy3

100 μg Cy3 conjugated

$570
PA1650-Dylight488

100 μg Dylight488 conjugated

$570
PA1650-Dylight550

100 μg Dylight550 conjugated

$570
PA1650-Dylight594

100 μg Dylight594 conjugated

$570
PA1650-FITC

100 μg FITC conjugated

$570
PA1650-HRP

100 μg HRP conjugated

$570
PA1650-APC

100 μg APC conjugated

$670
PA1650-PE

100 μg PE conjugated

$670
PA1650-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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