Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC, ICC/IF, FCM in Human;Mouse.
Anti-MCM2 Antibody Info At A Glance
|Applications:||Flow Cytometry, IF, IHC-P, ICC, WB|
CD1a is a transmembrane glycoproteins, which is structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1a mediates the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined.
|Product Name||Anti-MCM2 Antibody
See all MCM2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MCM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MCM2 information: Molecular Weight: 101896 MW; Subcellular Localization: Nucleus .|
|Cite This Product||Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1650)|
|Similar Products From
|Anti-MCM2 Antibody may replace the following items: sc 9839|sc 10771|sc 373702|sc 28551|sc 56322|sc 73572|sc 56321|sc 6680.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||Flow Cytometry, IF, IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2(889-904aa NKFSHDLKRKMILQQF), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MCM2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4171|
|Species Of This Entry||Human|
|Protein Name||DNA replication licensing factor MCM2|
|Alternative Names||MCM2|BM28; BM28MGC10606; CCNL 1; CCNL1; cdc19; CDCL1; CDCL1mitotin; D3S3194; EC 188.8.131.52; KIAA0030MITOTIN; MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae); MCM2; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); Mitotin|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Acetylation; Methylation; Biotinylation; Deacetylation; Ribosylation; Ubiquitination|
|Gene Location||3q21.3, on Chromasome 3, gene sequence: NC_000003.12 (127598411..127622436)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MCM2 (Source: Uniprot.org, NCBI)
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.|
|Interacting Proteins||AKAP8; ASF1A; CDC45; CENPA; HIST1H3A; HIST1H4A; L3MBTL1; MCM3; MCM5; MCM6; MCM7; MCMBP; PLK1; SNF8; SSRP1; TERF2IP|
|Research Areas||Cancer; Cell Biology; Cell Cycle and Replication; Cellular Markers; Core ESC-Like Genes; DNA Repair; DNA replication, Transcription, Translation and Splicing; Stem Cell Markers|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Malignant Paraganglionic Neoplasm; Malignant Squamous Cell Neoplasm; Adenocarcinoma; Dysplasia; Uterine Cervical Neoplasm; Neoplasm Metastasis; Cervical Intraepithelial Neoplasia; Cell Transformation, Neoplastic; Retinoblastoma; Precancerous Conditions; Intraepithelial Neoplasia; Carcinogenesis; Cell Invasion; Lung Neoplasms; Malignant Neoplasm Of Breast; Hyperplasia; Mammary Neoplasms|
|Related Pathways||Dna Replication; Cell Cycle; S Phase; Cell Proliferation; Mitosis; G1 Phase; Localization; Cell Division; Cell Growth; Cell Cycle Arrest; Pathogenesis; Dna Replication Initiation; Neurogenesis; Senescence; Cell Death; Interphase; Dna Repair; Regulation Of Dna Replication; Meiosis; Hyperphosphorylation|
|Background||MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.|
|Scientific References||PMID: 8175912 by Todorov I.T., et al. A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.|
PMID: 8258304 by Mincheva A., et al. The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21.
Our Boster Quality Guarantee for Anti-MCM2 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: SW620 Cell Lysate
Lane 2: PANC Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HELA Cell Lysate
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
ICC: A549 Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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