Product Info Summary
SKU: | PA1650 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCM2 Antibody
SKU/Catalog Number
PA1650
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM2 Antibody catalog # PA1650. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1650)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1650 is reactive to MCM2 in Human, Mouse
Applications
PA1650 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
125 kDa
Calculated molecular weight
101.896kDa
Background of MCM2
MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-MCM2 antibody, PA1650, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: PANC Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HELA Cell Lysate
Click image to see more details
Anti-MCM2 antibody, PA1650, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-MCM2 antibody, PA1650, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-MCM2 antibody, PA1650, ICC
ICC: A549 Cell
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Figure 5. IF analysis of MCM2 using anti-MCM2 antibody (PA1650).
MCM2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM2 Antibody (PA1650) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of HL-60 cells using anti-MCM2 antibody (PA1650).
Overlay histogram showing HL-60 cells stained with PA1650 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM2 Antibody (PA1650, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MCM2 (Source: Uniprot.org, NCBI)
Gene Name
MCM2
Full Name
DNA replication licensing factor MCM2
Weight
101.896kDa
Superfamily
MCM family
Alternative Names
BM28; BM28MGC10606; CCNL 1; CCNL1; cdc19; CDCL1; CDCL1mitotin; D3S3194; EC 3.6.4.12; KIAA0030MITOTIN; MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae); MCM2; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); Mitotin MCM2 BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 minichromosome maintenance complex component 2 DNA replication licensing factor MCM2|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCM2, check out the MCM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM2 Antibody (PA1650)
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No publications found for PA1650
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-MCM2 Antibody
Question
Is a blocking peptide available for product anti-MCM2 antibody (PA1650)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
We do provide the blocking peptide for product anti-MCM2 antibody (PA1650). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-24
Question
We have observed staining in mouse placenta uterus. Any tips? Is anti-MCM2 antibody supposed to stain placenta uterus positively?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Based on literature placenta uterus does express MCM2. Based on Uniprot.org, MCM2 is expressed in oocyte, colon carcinoma, bone marrow, brain, lung, lymph, muscle, placenta uterus, cervix carcinoma, prostate cancer, embryonic kidney, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-03-05
Question
Can you help my question with product PA1650, anti-MCM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1650 anti-MCM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-23
Question
I am interested in to test anti-MCM2 antibody PA1650 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-MCM2 antibody PA1650 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The products we sell, including anti-MCM2 antibody PA1650, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-12
Question
Is this PA1650 anti-MCM2 antibody reactive to the isotypes of MCM2?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of PA1650 anti-MCM2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2(889-904aa NKFSHDLKRKMILQQF), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
I see that the anti-MCM2 antibody PA1650 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-20
Question
We purchased anti-MCM2 antibody for WB on leukemic t-cell in the past. I am using human, and We intend to use the antibody for IHC next. I was wanting to use examining leukemic t-cell as well as embryonic kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
I took a look at the website and datasheets of our anti-MCM2 antibody and I see that PA1650 has been tested on human in both WB and IHC. Thus PA1650 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-01-31
Question
Does PA1650 anti-MCM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
It shows on the product datasheet, PA1650 anti-MCM2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-09
Question
Would anti-MCM2 antibody PA1650 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
According to the expression profile of cervix carcinoma, MCM2 is highly expressed in cervix carcinoma. So, it is likely that anti-MCM2 antibody PA1650 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2018-01-01
Question
I was wanting to use your anti-MCM2 antibody for IHC for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
It shows on the product datasheet, PA1650 anti-MCM2 antibody has been tested for IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-08
Question
We need using your anti-MCM2 antibody for g1/s transition of mitotic cell cycle studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
H. Miller
Verified customer
Asked: 2017-01-10
Answer
We appreciate your inquiry. This PA1650 anti-MCM2 antibody is tested on sw620 cell lysate, panc cell lysate, jurkat cell lysate, hela cell lysate, lung cancer tissue, intestinal cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-10
Question
Is there a BSA free version of anti-MCM2 antibody PA1650 available?
M. Krishna
Verified customer
Asked: 2016-01-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MCM2 antibody PA1650 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-01-14
Question
My boss were well pleased with the WB result of your anti-MCM2 antibody. However we have observed positive staining in prostate cancer nucleus. using this antibody. Is that expected? Could you tell me where is MCM2 supposed to be expressed?
D. Jackson
Verified customer
Asked: 2015-01-05
Answer
From what I have seen in literature, prostate cancer does express MCM2. Generally MCM2 expresses in nucleus. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2015-01-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-MCM2 antibody PA1650. Let me know if you need anything else.
K. Parker
Verified customer
Asked: 2015-01-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-02
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-MCM2 antibody PA1650. Please let me know if you require anything else.
K. Yang
Verified customer
Asked: 2014-09-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-22
Question
We are currently using anti-MCM2 antibody PA1650 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
N. Yang
Verified customer
Asked: 2014-03-03
Answer
The anti-MCM2 antibody (PA1650) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-03