Anti-AP-3 complex subunit mu-1 AP3M1 Antibody

Product Name

Anti-AP-3 complex subunit mu-1 AP3M1 Antibody

View all AP3M1 Antibodies

SKU/Catalog Number

A12628

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AP-3 complex subunit mu-1 AP3M1 Antibody (Catalog # A12628). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

AP3M1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-AP-3 complex subunit mu-1 AP3M1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12628)

Host

Rabbit

Contents

AP3M1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AP3M1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1. The immunogen is located within amino acids 340 - 390 of AP3M1.

Cross-reactivity

AP3M1 antibody may cross-react with AP3M2.

Reactive Species

A12628 is reactive to AP3M1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.9 kDa

Background of AP3M1

The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12628 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AP3M1

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