|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Mcm7 Antibody (Monoclonal, DCS-141)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Mcm7, minichromosome maintenance complex component 7 (MCM7) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Mcm7 Antibody (Monoclonal, DCS-141) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1058)|
|Immunogen||Recombinant human Mcm7.|
Assay Dilutions Overview
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Protein Target Info (Source: Uniprot.org)
|Protein Name||DNA helicase|
|Alternative Names||DNA helicase ;18.104.22.168 ;Mcm7 ;rCG_55961 ;|
|Subcellular Localization||Nucleus .|
|Molecular Weight||81063 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.|
|Background||Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.|
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