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| SKU | MA1058 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human |
| Clonality | Monoclonal |
| Clone Number | DCS-141 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | ICC, WB |
Overview
| Product Name | Anti-Mcm7 Antibody (Monoclonal, DCS-141) |
|---|---|
| SKU/Catalog Number | MA1058 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Mcm7, minichromosome maintenance complex component 7 (MCM7) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-Mcm7 Antibody (Monoclonal, DCS-141) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1058) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Recombinant human Mcm7. |
| Reactivity | Human |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.Images And Assay Conditions
Boster Kit Box
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q6AYN8 |
|---|---|
| Gene Name | MCM7 |
| Protein Name | DNA helicase |
| Alternative Names | DNA helicase ;3.6.4.12 ;Mcm7 ;rCG_55961 ; |
| Subcellular Localization | Nucleus . |
| Molecular Weight | 81063 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage. |
|---|---|
| Research Areas | Dna / Rna, Dna Synthesis, Epigenetics And Nuclear Signaling *You can search these to find other products in these research areas. |
| Background | Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1. |
Other Recommended Resources
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.