Anti-Mcm7 Antibody (Monoclonal, DCS-141)

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Datasheet

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SKU MA1058
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number DCS-141
Host Mouse
Ig Isotype Mouse IgG1
Applications ICC, WB

Overview

Product Name Anti-Mcm7 Antibody (Monoclonal, DCS-141)
SKU/Catalog Number MA1058
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Mcm7, minichromosome maintenance complex component 7 (MCM7) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-Mcm7 Antibody (Monoclonal, DCS-141) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1058)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Recombinant human Mcm7.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q6AYN8
Gene Name MCM7
Protein Name DNA helicase
Alternative Names DNA helicase ;3.6.4.12 ;Mcm7 ;rCG_55961 ;
Subcellular Localization Nucleus .
Molecular Weight 81063 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.
Research Areas Dna / Rna, Dna Synthesis, Epigenetics And Nuclear Signaling

*You can search these to find other products in these research areas.
Background Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.

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Order Product (MA1058)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships in 7-10 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.