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MCM2 antibody (M00374-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of SiHa cells using anti- MCM2 antibody (M00374-1).
Overlay histogram showing SiHa cells stained with M00374-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM2 Antibody (M00374-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with M00374-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM2 Antibody (M00374-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MCM2 Antibody Picoband® (monoclonal, 11C4)
MCM2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MCM2 antibody was positively detected by WB in:
human Hela whole cell, human CACO-2 whole cell, monkey COS-7 whole cell, human HL-60 whole cell, human HEK293 whole cell, human THP-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MCM2 antibody was positively detected by IHC in:
human rectal cancer tissue, human tonsil tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MCM2 antibody was positively detected by ICC/IF in:
Hela cell
MCM2 antibody was positively detected by IF in:
human tonsil tissue
MCM2 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
4μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Monkey
Tested
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MCM2 Antibody Picoband® (monoclonal, 11C4)
View all MCM2 Antibodies
View all MCM2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
11C4
Isotype
Mouse IgG1
Immunogen
E.coli-derived human MCM2 recombinant protein (Position: S393-R850).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
125 kDa
Calculated Molecular Weight
101.9 kDa
Background of MCM2
MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
Cite This Product
Anti-MCM2 Antibody Picoband® (monoclonal, 11C4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00374-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
99%
High Homology
Dog
97%
High Homology
Rat
97%
High Homology
Mouse
97%
High Homology
Cow
97%
High Homology
Sheep
96%
High Homology
Zebrafish
90%
High Homology
Drosophila
72%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MCM2 antibody
Immunohistochemistry (IHC) protocol for MCM2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MCM2 antibody
Immunofluorescence (IF) protocol for MCM2 antibody
Flow Cytometry (FC) protocol for MCM2 antibody
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Product Promise
We promise Anti-MCM2 Antibody Picoband® (monoclonal, 11C4) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00374-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026