Anti-MCM2 Antibody Picoband™

MCM2 antibody

Boster Bio Anti-MCM2 Antibody Picoband™ catalog # A00374-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00374-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-MCM2 Antibody Picoband™

View all MCM2 Antibodies

SKU/Catalog Number

A00374-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCM2 Antibody Picoband™ catalog # A00374-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00374-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MCM2 recombinant protein (Position: S393-R850).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00374-1 is reactive to MCM2 in Human, Mouse, Rat

Applications

A00374-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

125 kDa

Calculated molecular weight

101.896kDa

Background of MCM2

MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MCM2 (Source: Uniprot.org, NCBI)

Gene Name

MCM2

Full Name

DNA replication licensing factor MCM2

Weight

101.896kDa

Superfamily

MCM family

Alternative Names

BM28; BM28MGC10606; CCNL 1; CCNL1; cdc19; CDCL1; CDCL1mitotin; D3S3194; EC 3.6.4.12; KIAA0030MITOTIN; MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae); MCM2; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); Mitotin MCM2 BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 minichromosome maintenance complex component 2 DNA replication licensing factor MCM2|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MCM2, check out the MCM2 Infographic

MCM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MCM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MCM2 Antibody Picoband™

Question

We have been able to see staining in rat lymph. Any tips? Is anti-MCM2 antibody supposed to stain lymph positively?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

From what I have seen in literature lymph does express MCM2. From what I have seen in Uniprot.org, MCM2 is expressed in oocyte, colon carcinoma, bone marrow, brain, lung, lymph, muscle, placenta uterus, cervix carcinoma, prostate cancer, embryonic kidney, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-01-07

Question

We are currently using anti-MCM2 antibody A00374-1 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

The anti-MCM2 antibody (A00374-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-07

Question

We want using your anti-MCM2 antibody for activation of the pre-replicative complex studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

We appreciate your inquiry. This A00374-1 anti-MCM2 antibody is tested on human k562, k562 whole cell lysates, rat ovarian tissue lysates, mouse ovarian tissue lysates, nih3t3 whole cell lysates, a431 cells. It is guaranteed to work for ELISA, Flow Cytometry in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-30

Question

We were well pleased with the WB result of your anti-MCM2 antibody. However we have been able to see positive staining in brain nucleus. using this antibody. Is that expected? Could you tell me where is MCM2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

Based on literature, brain does express MCM2. Generally MCM2 expresses in nucleus. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-06-22

Question

We ordered your anti-MCM2 antibody for Flow Cytometry on colon carcinoma a few years ago. I am using human, and We are going to use the antibody for ELISA next. Our lab want to know about examining colon carcinoma as well as bone marrow in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

R. Yang

Verified customer

Asked: 2015-04-15

Answer

I have checked the website and datasheets of our anti-MCM2 antibody and it seems that A00374-1 has been tested on human in both Flow Cytometry and ELISA. Thus A00374-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-04-15

Order DetailsPrice
A00374-1

100μg

$370
A00374-1-10ug

10μg sample (liquid)

$99
A00374-1-Biotin

100 μg Biotin conjugated

$570
A00374-1-Cy3

100 μg Cy3 conjugated

$570
A00374-1-Dylight488

100 μg Dylight488 conjugated

$570
A00374-1-Dylight550

100 μg Dylight550 conjugated

$570
A00374-1-Dylight594

100 μg Dylight594 conjugated

$570
A00374-1-FITC

100 μg FITC conjugated

$570
A00374-1-HRP

100 μg HRP conjugated

$570
A00374-1-APC

100 μg APC conjugated

$670
A00374-1-PE

100 μg PE conjugated

$670
A00374-1-carrier-free

Carrier Free

$370

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