Anti-Mdm2-binding protein MTBP Antibody

Product Name

Anti-Mdm2-binding protein MTBP Antibody

View all MTBP Antibodies

SKU/Catalog Number

A08842

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Mdm2-binding protein MTBP Antibody (Catalog # A08842). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

MTBP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Mdm2-binding protein MTBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08842)

Host

Rabbit

Contents

MTBP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MTBP antibody was raised against a 18 amino acid peptide near the center of human MTBP. The immunogen is located within amino acids 110 - 160 of MTBP.

Reactive Species

A08842 is reactive to MTBP in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

102.2 kDa

Background of MTBP

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A08842 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MTBP antibody can be used for detection of MTBP by Western blot at 1 to 2 μg/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MTBP

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis