Product Info Summary
SKU: | A05399-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MED17 Antibody Picoband™
SKU/Catalog Number
A05399-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED17 Antibody Picoband™ catalog # A05399-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MED17 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05399-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MED17 recombinant protein (Position: V4-E561).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05399-2 is reactive to MED17 in Human
Applications
A05399-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
73 kDa
Calculated molecular weight
72.89kDa
Background of MED17
Mediator of RNA polymerase II transcription subunit 17 is an enzyme that in humans is encoded by the MED17 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MED17 using anti-MED17 antibody (A05399-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED17 antigen affinity purified polyclonal antibody (Catalog # A05399-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED17 at approximately 73 kDa. The expected band size for MED17 is at 73 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of U251 cells using anti-MED17 antibody (A05399-2).
Overlay histogram showing U251 cells stained with A05399-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED17 Antibody (A05399-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MED17 (Source: Uniprot.org, NCBI)
Gene Name
MED17
Full Name
Mediator of RNA polymerase II transcription subunit 17
Weight
72.89kDa
Superfamily
Mediator complex subunit 17 family
Alternative Names
Activator-recruited cofactor 77 kDa component; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP6FLJ10812; CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD); DRIP77; DRIP80ARC77; mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component; mediator of RNA polymerase II transcription subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Transcriptional coactivator CRSP77; Trap80; TRAP80CRSP complex subunit 6 MED17 CRSP6, CRSP77, DRIP80, SRB4, TRAP80 mediator complex subunit 17 mediator of RNA polymerase II transcription subunit 17|ARC77|CRSP complex subunit 6|activator-recruited cofactor 77 kDa component|cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa|epididymis secretory sperm binding protein|thyroid hormone receptor-associated protein complex 80 kDa component|transcriptional coactivator CRSP77|vitamin D3 receptor-interacting protein complex 80 kDa component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED17, check out the MED17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED17 Antibody Picoband™ (A05399-2)
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No publications found for A05399-2
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