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Product Info Summary
| SKU: | A17100 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GEMC1 GMNC Antibody
SKU/Catalog Number
A17100
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GEMC1 GMNC Antibody (Catalog # A17100). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
GEMC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GEMC1 GMNC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17100)
Host
Rabbit
Contents
GEMC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GEMC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human GEMC1. The immunogen is located within amino acids 130 - 180 of GEMC1.
Reactive Species
A17100 is reactive to GEMC1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
37.9 kDa
Background of GEMC1
The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17100 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GEMC1 in mouse heart tissue lysate with GEMC1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Specific Publications For Anti-GEMC1 GMNC Antibody (A17100)
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