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- Table of Contents
Cycs antibody (A03529) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytochrome c/CYCS Antibody (A03529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cytochrome c/CYCS was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytochrome c/CYCS Antibody (A03529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Cytochrome C/Cycs Antibody Picoband®
Cycs antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
Cycs antibody was positively detected by WB in:
human HL-60 whole cell, human K562 whole cell, human U-937 whole cell, human U20S whole cell, human T-47D whole cell, human PC-3 whole cell, human CACO-2 whole cell, rat brain tissue, rat heart tissue, rat kidney tissue, rat C6 whole cell, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cycs antibody was positively detected by IHC in:
mouse brain tissue, mouse intestine tissue, rat brain tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytochrome C/Cycs Antibody Picoband®
View all CYCS Antibodies
View all CYCS Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Cytochrome C recombinant protein (Position: G2-E105). Mouse Cytochrome C shares 91.3% and 100% amino acid (aa) sequence identity with human and rat Cytochrome C, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
11.6 kDa
Background of Cycs
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Cite This Product
Anti-Cytochrome C/Cycs Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03529)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
100%
High Homology
Sheep
98%
High Homology
Cow
97%
High Homology
Dog
96%
High Homology
Monkey
92%
High Homology
Human
91%
High Homology
Zebrafish
90%
High Homology
Drosophila
85%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cycs antibody
Immunohistochemistry (IHC) protocol for Cycs antibody
Publications
Application:
Species:
Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment
Published: 2025-07-14
Journal: Cancer Nanotechnology
Application: WB
Impact Factor: 4.80
Species: Human
New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress
Published: 2024-03-16
Journal: POULTRY SCIENCE
Application: WB
Impact Factor: 3.80
Species: Chicken
PMID: 38583309
Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity
Published: 2024-02-29
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 7.70
Species: Human
PMID: 38423906
Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis
Published: 2024-02-26
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 1.50
Species: Chicken
PMID: 38427138
Polystyrene microplastics mediate inflammatory responses in the chicken thymus by Nrf2/NF-κB pathway and trigger autophagy and apoptosis
Published: 2023-04-29
Journal: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Application: WB
Impact Factor: 4.30
Species: Chicken
PMID: 37127111
Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism
Published: 2019-10-01
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Rat
PMID: 31702040
Mitochondria-Targeted and Resveratrol-Loaded Dual-Function Titanium Disulfide Nanosheets for Photothermal-Triggered Tumor Chemotherapy
Published: 2019-06-21
Journal: Nanoscale Research Letters
Application: WB
Impact Factor: 3.16
Species: Mouse
PMID: 31227943
Rhodiola crenulata attenuates apoptosis and mitochondrial energy metabolism disorder in rats with hypobaric hypoxia-induced brain injury by regulating the HIF-1α/microRNA 210/ISCU1/2(COX10) signaling pathway
Published: 2019-03-13
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 3.41
Species: Rat
PMID: 30878546
Establishment and evaluation of a simulated high‑altitude hypoxic brain injury model in SD rats
Published: 2019-02-05
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Rat
PMID: 30720143
Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes
Published: 2018-04-11
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.56
Species: Human
PMID: 29626474
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Product Promise
We promise Anti-Cytochrome C/Cycs Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03529
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026