Anti-Cytochrome C/Cycs Antibody Picoband™

Boster Bio Anti-Cytochrome C/Cycs Antibody Picoband™ catalog # A03529. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: A03529
Size: 100ug/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Cytochrome C/Cycs Antibody Picoband™

See all Cytochrome c products

SKU/Catalog Number

A03529

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome C/Cycs Antibody Picoband™ catalog # A03529. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome C/Cycs Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03529)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse Cytochrome C recombinant protein (Position: G2-E105). Mouse Cytochrome C shares 91.3% and 100% amino acid (aa) sequence identity with human and rat Cytochrome C, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A03529 is reactive to Cycs in Human, Mouse, Rat

Applications

A03529 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat,

Validation Images & Assay Conditions

Gene/Protein Information For Cycs (Source: Uniprot.Org, NCBI)

Uniprot ID

P62897

Gene ID

13063

Gene Name

Cycs

Full Name

Cytochrome c, somatic

Weight

11.605kDa

Superfamily

cytochrome c family

Alternative Names

CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4 Cycs|cytochrome c, somatic|cytochrome c, somatic

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cycs, check out the Cycs Infographic

Cycs infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cycs: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A03529 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang T,Chen Y,Cai J,Pan M,Sun Q,Zhang J,Sun C.SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes.Oxid Med Cell Longev.2020 Jul 13;2020:3742542.doi:10.1155/2020/3742542.PMID:32733634;PMCI
Species: Mouse
A03529 usage in article: APP:IF, SAMPLE:ADIPOCYTES, DILUTION:1:100

Pioglitazone improves mitochondrial function in the remnant kidney and protects against renal fibrosis in 5/6 nephrectomized rats

Neuroprotective Effect of Tea Polyphenols on Oxyhemoglobin Induced Subarachnoid Hemorrhage in Mice

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

pH-sensitive micelles for the intracellular co-delivery of curcumin and Pluronic L61 unimers for synergistic reversal effect of multidrug resistance

Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells

Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway

Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress

N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells

HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma

FABP4 reversed the regulation of leptin on mitochondrial fatty acid oxidation in mice adipocytes

Guo X, Fu Y, Wang Z, Wang T, Li C, Huang T, Gao F, Li C. Oxid Med Cell Longev. 2018 Mar 25;2018:4950705. doi: 10.1155/2018/4950705. eCollection 2018. Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition aga...

You F, Li Q, Jin G, Zheng Y, Chen J, Yang H. BMC Neurosci. 2017 Jan 12;18(1):12. doi: 10.1186/s12868-016-0329-9. Genistein protects against Aβ25–35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers

Bai J, Yang BJ, Luo X. Oncol Lett. 2018 Apr;15(4):5227-5235. doi: 10.3892/ol.2018.7938. Epub 2018 Feb 2. Effects of 5-hydroxy-4

Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...

Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.

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16 Customer Q&As for Anti-Cytochrome C/Cycs Antibody Picoband™

Question

I see that the anti-Cytochrome C/Cycs antibody A03529 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-24

Question

We are currently using anti-Cytochrome C/Cycs antibody A03529 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

The anti-Cytochrome C/Cycs antibody (A03529) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-24

Question

We ordered your anti-Cytochrome C/Cycs antibody for IHC on kidney in the past. I am using rat, and We intend to use the antibody for WB next. I am interested in examining kidney as well as cerebellum in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

I have checked the website and datasheets of our anti-Cytochrome C/Cycs antibody and I see that A03529 has been validated on rat in both IHC and WB. Thus A03529 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-13

Question

Is this A03529 anti-Cytochrome C/Cycs antibody reactive to the isotypes of CYCS?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

The immunogen of A03529 anti-Cytochrome C/Cycs antibody is E. coli-derived mouse Cytochrome C recombinant protein (Position: G2-E105). Mouse Cytochrome C shares 91.3% and 100% amino acid (aa) sequence identity with human and rat Cytochrome C, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-17

Question

My team were content with the WB result of your anti-Cytochrome C/Cycs antibody. However we have seen positive staining in brain mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

From literature, brain does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-12-11

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Cytochrome C/Cycs antibody A03529. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-14

Question

I was wanting to use your anti-Cytochrome C/Cycs antibody for IHC for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?

E. Edwards

Verified customer

Asked: 2019-10-04

Answer

As indicated on the product datasheet, A03529 anti-Cytochrome C/Cycs antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-04

Question

I was wanting to use using your anti-Cytochrome C/Cycs antibody for cytochrome c to oxygen studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

I appreciate your inquiry. This A03529 anti-Cytochrome C/Cycs antibody is tested on rat heart tissue, intestine tissue, tissue lysate, brain tissue, mouse heart tissue, human hela, hela whole cell lysate, 293t whole cell lysate, hepg2 whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-17

Question

Will anti-Cytochrome C/Cycs antibody A03529 work for IHC with brain?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

According to the expression profile of brain, CYCS is highly expressed in brain. So, it is likely that anti-Cytochrome C/Cycs antibody A03529 will work for IHC with brain.

Boster Scientific Support

Answered: 2019-06-10

Question

I have a question about product A03529, anti-Cytochrome C/Cycs antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-04-16

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03529 anti-Cytochrome C/Cycs antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-16

Question

Is a blocking peptide available for product anti-Cytochrome C/Cycs antibody (A03529)?

L. Gonzalez

Verified customer

Asked: 2019-04-11

Answer

We do provide the blocking peptide for product anti-Cytochrome C/Cycs antibody (A03529). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-11

Question

We have been able to see staining in human kidney. Do you have any suggestions? Is anti-Cytochrome C/Cycs antibody supposed to stain kidney positively?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

Based on literature kidney does express CYCS. Based on Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin, rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-12-27

Question

I am interested in to test anti-Cytochrome C/Cycs antibody A03529 on mouse brain for research purposes, then I may be interested in using anti-Cytochrome C/Cycs antibody A03529 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Li

Verified customer

Asked: 2017-11-24

Answer

The products we sell, including anti-Cytochrome C/Cycs antibody A03529, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-24

Question

Is there a BSA free version of anti-Cytochrome C/Cycs antibody A03529 available?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome C/Cycs antibody A03529 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-27

Question

Does A03529 anti-Cytochrome C/Cycs antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

O. Dhar

Verified customer

Asked: 2014-07-10

Answer

As indicated on the product datasheet, A03529 anti-Cytochrome C/Cycs antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-07-10

Question

Please see the WB image, lot number and protocol we used for brain using anti-Cytochrome C/Cycs antibody A03529. Please let me know if you require anything else.

E. Zhao

Verified customer

Asked: 2014-04-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-18

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A03529
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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