Anti-MEGF9/Egfl5 Antibody

MEGF9 antibody

Boster Bio Anti-MEGF9/Egfl5 Antibody (Catalog # A15544). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A15544
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-MEGF9/Egfl5 Antibody

View all MEGF9 Antibodies

SKU/Catalog Number

A15544

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MEGF9/Egfl5 Antibody (Catalog # A15544). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MEGF9/Egfl5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15544)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MEGF9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A15544 is reactive to MEGF9 in Human, Mouse

Applications

A15544 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

62.984kDa

Background of MEGF9

Potassium channel subunit. Modulates channel activity and reduces the ion flow By similarity.

Hirosawa M., DNA Res. 6:329-336(1999).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For MEGF9 (Source: Uniprot.org, NCBI)

Gene Name

MEGF9

Full Name

Multiple epidermal growth factor-like domains protein 9

Weight

62.984kDa

Alternative Names

EGFL5; EGFL5KIAA0818; EGF-like protein 5; EGF-like-domain, multiple 5; Epidermal growth factor-like protein 5; MEGF9; Multiple EGF-like domains protein 9; multiple EGF-like-domains 9; multiple epidermal growth factor-like domains protein 9 MEGF9 EGFL5 multiple EGF like domains 9 multiple epidermal growth factor-like domains protein 9|EGF-like-domain, multiple 5|epidermal growth factor-like protein 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MEGF9, check out the MEGF9 Infographic

MEGF9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MEGF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-MEGF9/Egfl5 Antibody

Question

Do you have a BSA free version of anti-MEGF9/Egfl5 antibody A15544 available?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEGF9/Egfl5 antibody A15544 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for trigeminal ganglion using anti-MEGF9/Egfl5 antibody A15544. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-22

Question

See below the WB image, lot number and protocol we used for trigeminal ganglion using anti-MEGF9/Egfl5 antibody A15544. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-11

Question

My question regards to test anti-MEGF9/Egfl5 antibody A15544 on human trigeminal ganglion for research purposes, then I may be interested in using anti-MEGF9/Egfl5 antibody A15544 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

H. Brown

Verified customer

Asked: 2019-05-13

Answer

The products we sell, including anti-MEGF9/Egfl5 antibody A15544, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-13

Question

Would A15544 anti-MEGF9/Egfl5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Roberts

Verified customer

Asked: 2017-03-22

Answer

You can see on the product datasheet, A15544 anti-MEGF9/Egfl5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-03-22

Question

Can you help my question with product A15544, anti-MEGF9/Egfl5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Jones

Verified customer

Asked: 2013-04-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15544 anti-MEGF9/Egfl5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-04-16

Question

I see that the anti-MEGF9/Egfl5 antibody A15544 works with WB, what is the protocol used to produce the result images on the product page?

N. Zhang

Verified customer

Asked: 2013-04-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-15

Order DetailsPrice
A15544-100uL

100uL

$360

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A15544
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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