This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
MAP2K3 antibody (PB9763) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of CACO-2 cells using anti-MEK3 antibody (PB9763).
Overlay histogram showing CACO-2 cells stained with PB9763 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK3 Antibody (PB9763, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with PB9763 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK3 Antibody (PB9763, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-MEK3/MAP2K3 Antibody Picoband®
MAP2K3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MAP2K3 antibody was positively detected by WB in:
human Hela whole cell, rat kidney tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MAP2K3 antibody was positively detected by IHC in:
human liver cancer tissue, human ovarian cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MAP2K3 antibody was positively detected by ICC/IF in:
CACO-2 cell
MAP2K3 antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MEK3/MAP2K3 Antibody Picoband®
View all MAP2K3 Antibodies
View all MAP2K3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3, identical to the related mouse sequence.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
39.3 kDa
Background of MAP2K3
Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. And this kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al. (1997) localized the MAP2K3 gene to 17q11.2.
Cite This Product
Anti-MEK3/MAP2K3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9763)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Rabbit
95%
High Homology
Zebrafish
62%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MAP2K3 antibody
Immunohistochemistry (IHC) protocol for MAP2K3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MAP2K3 antibody
Flow Cytometry (FC) protocol for MAP2K3 antibody
Publications
Application:
Species:
MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3
Published: 2013-10-10
Journal: BMC CANCER
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 24112539
Review
Have you used Anti-MEK3/MAP2K3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-MEK3/MAP2K3 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9763
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 29, 2026