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Product Info Summary
| SKU: | M01262-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI10E5]
SKU/Catalog Number
M01262-1
Size
100 µl
Description
Boster Bio Anti-SILV (Melanoma gp100) mouse monoclonal antibody, clone OTI10E5 (formerly 10E5). Catalog# M01262-1. Tested in IF, IHC, WB. This antibody reacts with Human, Monkey, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI10E5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01262-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI10E5
Isotype
IgG2b
Immunogen
Full-length protein expressed in 293T cell transfected with human SILV expression vector
Reactive Species
M01262-1 is reactive to PMEL in Dog, Human, Monkey, Rat
Calculated molecular weight
70.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01262-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000 IHC 1:50 IF 1:100 Flow Cytometry 1:500
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SILV (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SILV.
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-SILV monoclonal antibody at 1:200 dilution.
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SILV monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-SILV mouse monoclonal antibody (M01262-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SILV.
Specific Publications For Anti-Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI10E5] (M01262-1)
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