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- Table of Contents
BDNF antibody (M00035-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 17 Publications
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Anti-BDNF Rabbit Monoclonal Antibody
BDNF antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
BDNF antibody was positively detected by IHC in:
Human esophageal carcinoma tissue, Human lung tissue, Rat stomach tissue, Mouse heart tissue, Rat pancreas tissue, human testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Recommend
Tested
Recommend
Recommend
Rat
Recommend
Tested
Recommend
Recommend
Mouse
Recommend
Tested
Recommend
Recommend
Product Information
Product Name
Anti-BDNF Rabbit Monoclonal Antibody
View all BDNF Antibodies
View all BDNF Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BEH-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BDNF
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
27.8 kDa
Cite This Product
Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-1)
Protocols
Immunohistochemistry (IHC) protocol for BDNF antibody
Publications
Application:
Species:
Identification and Validation of Antidepressant Small Molecules Using Bioinformatics and Mouse Depression Models
Published: 2025-08-20
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40859969
Low-Frequency (1 Hz) Repetitive Trans-Spinal Magnetic Stimulation Attenuates NLRP3-Driven Neuroinflammation and Enhances Motor Recovery in Spinal Cord-Injured Mice
Published: 2025-08-18
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 40873779
The Integration Methodology of Metabolomics and Bioinformatics to Investigate the Mechanism of the Jiawei Erzhi Formula in Treating Postmenopausal Osteoporosis Based on the Kidney-Bone Axis
Published: 2025-08-06
Journal: Separation Science Plus
Application: WB
Impact Factor: 1.60
Species: Rat
Buyang Huanwu Decoction Promotes Recovery after Spinal Cord Injury by Regulating cAMP/PKA/NF-κB p65 Pathway
Published: 2025-05-15
Journal: Chinese Journal of Integrative Medicine
Application: IHC
Impact Factor: 2.50
Species: Rat
PMID: 40372579
Revealing the effective components and mechanism of Zhimu-Huangbai herb-pair in the treatment of Alzheimer's disease
Published: 2025-03-26
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 5.40
Species: Mouse
PMID: 40154898
Chrysin modulates the BDNF/TrkB/AKT/Creb neuroplasticity signaling pathway: Acting in the improvement of cognitive flexibility and declarative, working and aversive memory deficits caused by hypothyroidism in C57BL/6 female mice
Published: 2024-12-19
Journal: NEUROSCIENCE
Application: WB
Impact Factor: 2.90
Species: Mouse
PMID: 39709060
Neonatal Maternal Separation Impairs Cognitive Function and Synaptic Plasticity in Adult Male CD-1 Mice
Published: 2024-11-09
Journal: IBRO Neuroscience Reports
Application: WB
Impact Factor: 2.00
Species: Mouse
PMID: 39629017
Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model
Published: 2024-07-29
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: IHC
Impact Factor: 6.20
Species: Rat
PMID: 39073571
Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease
Published: 2024-05-23
Journal: ACS Chemical Neuroscience
Application: IHC
Impact Factor: 4.10
Species: Mouse
PMID: 38780450
Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance
Published: 2024-01-15
Journal: MOLECULAR NEUROBIOLOGY
Application: WB,IHC,IF
Impact Factor: 4.60
Species: Rat
PMID: 38221533
The hippocampal FTO-BDNF-TrkB pathway is required for novel object recognition memory reconsolidation in mice
Published: 2023-11-14
Journal: Translational Psychiatry
Application: WB
Impact Factor: 6.80
Species: Mouse
PMID: 37963912
Engineering brain-derived neurotrophic factor mRNA delivery for the treatment of Alzheimer’s disease
Published: 2023-04-23
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF,IHC
Impact Factor: 15.10
Species: Human,Mouse
Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction
Published: 2021-11-04
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB,IF
Impact Factor: 5.28
Species: Rat
PMID: 34735150
Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats
Published: 2021-02-23
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 2.95
Species: Rat
PMID: 33649786
Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor
Published: 2017-10-02
Journal: CELL BIOCHEMISTRY AND FUNCTION
Application: IF
Impact Factor: 2.19
Species: Mouse
PMID: 28967682
Curcumin improves spatial memory impairment induced by human immunodeficiency virus type 1 glycoprotein 120 V3 loop peptide in rats
Published: 2009-04-05
Journal: LIFE SCIENCES
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 19345695
Application of bFGF and BDNF to Improve Angiogenesis and Cardiac Function
Published: 2006-08-14
Journal: JOURNAL OF SURGICAL RESEARCH
Application: IHC
Impact Factor: 1.96
Species: Dog
PMID: 16904693
Review
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1 Review For Anti-BDNF Rabbit Monoclonal Antibody
The Anti-BDNF antibody (M00035-1) produced a clear, specific band with low background in mouse hippocampal tissue by Western blot, demonstrating excellent sensitivity and reliable performance.
| SKU | M00035-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse hippocampal tissue |
| Sample Processing Description | The left hippocampus was dissected from normal mouse brains, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | BDNF Rabbit Monoclonal Antibody |
| Primary Incubation | 1:4000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | Brain-derived neurotrophic factor (BDNF) is a secreted protein that plays a critical role in neuronal survival, growth, and synaptic plasticity. In this study, hippocampal tissues from two normal mice were used to evaluate the performance of the Anti-BDNF antibody. The results showed a clear target band at the expected molecular weight, demonstrating that the antibody performs reliably in Western blot. |
Yajun Qiao, Qinghai University
Verified customer
Submitted 2026-07-06
Product Promise
We promise Anti-BDNF Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00035-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026