Anti-BDNF Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.

Western blot analysis of extracts of Human cerebellum lysate, using BDNF antibody.

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded human testis, using BDNF Antibody .

Immunofluorescent analysis using the Antibody at 1:500 dilution.

Immunofluorescent analysis of HeLa cells, using BDNF Antibody.

The m6A-dependent mechanism of FTO in regulating the BDNF-TrkB pathway. A Western blotting shows the relative protein expression levels of FTO, BDNF, and TrkB in Control and MA treated HT22 cells ( n = 3); ** P < 0.01, * P < 0.05. B RT-PCR shows the relative levels of BDNF mRNA in Control and MA treated HT22 cells ( n = 3); *** P < 0.001. C Western blotting shows the relative protein expression levels of BDNF in LV-Vehicle and LV-FTO HT22 cells ( n = 3). ** P < 0.01, * P < 0.05. D RT-PCR shows the relative levels of BDNF mRNA in LV-Vehicle and LV-FTO HT22 cells ( n = 3); *** P < 0.001. E Prediction of m6A modification site on BDNF mRNA. F RT-PCR shows the m6A modification levels of BDNF mRNA in LV-Vehicle and LV-FTO cells ( n = 5). * P < 0.05. G Schematic of the role and mechanisms of FTO in NOR memory reconsolidation. The reactivation of NOR memory increased hippocampal FTO expression, which decrease the m6A level of BDNF mRNA and increased the protein expression of BDNF. BDNF binds to the high-affinity receptor TrkB to promote the NOR memory reconsolidation. The FTO inhibitor MA and TrkB antagonist ANA-12 disrupt NOR memory reconsolidation via targeting the FTO-BDNF-TrkB pathway.
Index in PubMed under a CC BY license. PMID: 37963912

FTO mediated the BDNF-TrkB pathway in NOR memory reconsolidation. A Schematic of the experimental design for NOR memory RA and sampling. B Representative BDNF blots of each group. C Quantitative analysis of BDNF expression in WB. N = 3. *** P = 0.0004, ** P = 0.0036. D Schematic of the experimental design for the effects of ANA-12, a TrkB antagonist, administered immediately after RA on NOR memory performance. E Total exploration times in Test 1 and Test 2. F DI of each group in Test 1 and Test 2. ** P = 0.0013, *** P = 0.0006. G Distance of travelled during the tests. H Schematic of the experimental design for the effects of 7,8-dihydroxyflavone (7,8-DHF), a TrkB agonist, on MA mediated disruption of NOR memory reconsolidation. I Total exploration times in Test 1 and Test 2. J DI of each group in Test 1 and Test 2. * P = 0.0287, DI of vehicle + MA group between Test 1 and Test 2; * P = 0.0216, DI between vehicle + MA group and vehicle + 7,8-DHF group in Test 2. K Distance of travelled during the tests.
Index in PubMed under a CC BY license. PMID: 37963912