Anti-Mesothelin MSLN Monoclonal Antibody

Product Name

Anti-Mesothelin MSLN Monoclonal Antibody

View all Mesothelin Antibodies

SKU/Catalog Number

M03266

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Mesothelin MSLN Monoclonal Antibody (Catalog # M03266). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Mesothelin MSLN Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03266)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: MN-1 IgG2a

Isotype

IgG2a

Immunogen

This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M03266 is reactive to MSLN in Human

Applications

M03266 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

68.986kDa

Background of Mesothelin

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:800
IHC: 1:100
WB: 1:1,000

Validation Images & Assay Conditions

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Immunohistochemistry using mouse anti-mesothelin monoclonal antibody (Catalog # M03266) to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.

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Western blotting using mouse anti-mesothelin monoclonal antibody (Catalog # M03266) at 1

Gene/Protein Information For MSLN (Source: Uniprot.org, NCBI)

Alternative Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein MSLN MPF, SMRP mesothelin mesothelin|CAK1 antigen|megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein

For more info on MSLN, check out the MSLN Infographic

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In this infographic, you will see the following information for MSLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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