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- Table of Contents
TFPI2 antibody (A03697-1) performs well in Flow Cytometry, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of U87 cells using anti-TFPI2 antibody (A03697-1).
Overlay histogram showing U87 cells stained with A03697-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFPI2 Antibody (A03697-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with A03697-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFPI2 Antibody (A03697-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TFPI2 Antibody Picoband®
TFPI2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IHC, IHC-F, ICC, WB |
Tested Applications
TFPI2 antibody was positively detected by WB in:
mouse spleen tissue, HELA whole Cell, human placenta tissue, MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TFPI2 antibody was positively detected by IHC in:
human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TFPI2 antibody was positively detected by FC in:
U87 cell, A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
Mouse
Tested
Recommend
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TFPI2 Antibody Picoband®
View all TFPI2 Antibodies
View all TFPI2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa, 35 kDa
Calculated Molecular Weight
26.9 kDa
Background of TFPI2
Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.
Cite This Product
Anti-TFPI2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03697-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
72%
Moderate Homology
Cow
69%
Low homology
Sheep
69%
Low homology
Mouse
44%
Low homology
Rat
44%
Low homology
Zebrafish
39%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TFPI2 antibody
Immunohistochemistry (IHC) protocol for TFPI2 antibody
Flow Cytometry (FC) protocol for TFPI2 antibody
Publications
Application:
Species:
TFPI2 suppresses the interaction of TGF-β2 pathway regulators to promote endothelial–mesenchymal transition in diabetic nephropathy
Published: 2022-02-10
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB,IHC,IF
Impact Factor: 5.49
Species: Mouse,Human
PMID: 35157852
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Product Promise
We promise Anti-TFPI2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03697-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026