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- Table of Contents
MMP16 antibody (PA1123) performs well in IHC, IHC-F, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of MMP16 using anti-MMP16 antibody (PA1123).
MMP16 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
MMP16 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband®
MMP16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC, IHC-F, WB |
Tested Applications
MMP16 antibody was positively detected by WB in:
human heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MMP16 antibody was positively detected by IHC in:
human rectal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MMP16 antibody was positively detected by IHC-F in:
human placenta tissue, rat intestine tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband®
View all MMP16 Antibodies
View all MMP16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
69.5 kDa
Background of MMP16
The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.
Cite This Product
Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1123)
Protocols
Western Blot protocol for MMP16 antibody
Immunohistochemistry (IHC) protocol for MMP16 antibody
Publications
Application:
Species:
miRNA 146b-5p protects against atherosclerosis by inhibiting vascular smooth muscle cell proliferation and migration
Published: 2020-10-21
Journal: Epigenomics
Application: WB
Impact Factor: 4.11
Species: Mouse
PMID: 33084403
MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma
Published: 2016-06-12
Journal: Cancer Medicine
Application: WB,IHC
Impact Factor: 2.92
Species: Human
PMID: 27292876
Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters
Published: 2015-01-01
Journal: BIO-MEDICAL MATERIALS AND ENGINEERING
Application: WB
Impact Factor: 1.09
Species: Rat
PMID: 26405980
Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16
Published: 2011-08-07
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: WB
Impact Factor: 0.41
Species: Human
PMID: 21823013
MMP-14 can serve as a prognostic marker in patients with supraglottic cancer
Published: 2009-03-13
Journal: EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
Application: IHC
Impact Factor: 0.84
Species: Human
PMID: 19283401
microRNA-146b inhibits glioma cell migration and invasion by targeting MMPs
Published: 2009-03-03
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 2.49
Species: Human
PMID: 19265686
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Product Promise
We promise Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® performs excellently in relevant applications for IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1123
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026