Anti-Matrix metalloproteinase-16 MMP16 Antibody
Rabbit IgG polyclonal antibody for Matrix metalloproteinase-16(MMP16) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. Cited in 3 publication(s).
Anti-Matrix metalloproteinase-16 MMP16 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Matrix metalloproteinase-16 MMP16 Antibody
See all MMP-16/MT3-MMP primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MT3 MMP/MMP16 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MT3 MMP/MMP16 information: Molecular Weight: 69521 MW; Subcellular Localization: Isoform Long: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells; Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.|
|Cite This Product||Anti-Matrix metalloproteinase-16 MMP16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1123)|
|Similar Products From
|Anti-Matrix metalloproteinase-16 MMP16 Antibody may replace the following items: sc 46325|sc 46324|sc 46320|sc 46318|sc 515004|sc 515003.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16(582-598aa, YTVFQFKRKGTPRHILY), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MMP16 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4325|
|Species Of This Entry||Human|
|Protein Name||Matrix metalloproteinase-16|
|Superfamily||peptidase M10A family|
|Alternative Names||MMP-16/MT3-MMP|chromosome 8 open reading frame 57; DKFZp761D112; EC 3.4.24; EC 3.4.24.-; EC 18.104.22.168; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16 (membrane-inserted); matrix metalloproteinase-16; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP16; MMP-16; MMPX2; MMP-X2; MT3MMP; MT3-MMP; MT3-MMPC8orf57; MT-MMP 3; MT-MMP2; MTMMP3; MT-MMP3; Putative transmembrane protein C8orf57|
|Post Tranlational Modifications||Cleavage|
|Subcellular Localization||[Isoform Long]: Cell membrane; Single-pass type I membrane protein; Extracellular side. Localized at the cell surface of melanoma cells.; [Isoform Short]: Secreted, extracellular space, extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.|
|Gene Location||8q21.3, on Chromasome 8, gene sequence: NC_000008.11 (88032009..88327483, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MMP16 (Source: Uniprot.org, NCBI)
|Protein Function||Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.|
|Tissue Specificity||Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.|
|Related Diseases||Neoplasms; Cell Invasion; Malignant Neoplasms; Neoplasm Invasiveness; Carcinoma; Neoplasm Metastasis; Glioma; Malignant Neoplasm Of Breast; Mammary Neoplasms; Dysplasia; Adenocarcinoma; Malignant Paraganglionic Neoplasm; Malignant Squamous Cell Neoplasm; Metastatic Malignant Neoplasm To The Lymph Node; Melanoma; Fibrosarcoma; Tumor Angiogenesis; Tumor Progression; Bronchopulmonary Dysplasia|
|Related Pathways||Proteolysis; Cell Migration; Localization; Tube Formation; Tissue Remodeling; Angiogenesis; Cell Adhesion; Endochondral Ossification; Gland Development; Lung Development; Metaphase; Ossification; Mammary Gland Development; Chondrocyte Hypertrophy; Drug Resistance; Endothelial Cell Migration; Mrna Stabilization; Mrna Processing; Invasive Growth; Pathogenesis|
|Background||The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.|
|Scientific References||PMID: 7559440 by Takino T., et al. Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library. MT-MMPs form a unique membrane-type subclass in the MMP family.|
PMID: 9396633 by Matsumoto S., et al. Identification of soluble type of membrane-type matrix metalloproteinase-3 formed by alternatively spliced mRNA.
Our Boster Quality Guarantee for Anti-Matrix metalloproteinase-16 MMP16 Antibody covers its use in the following applications.Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
MMP16 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (Catalog # PA1123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP16 at approximately 53KD. The expected band size for MMP16 is at 69KD or 53KD.
MMP16 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
MMP16 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Total number of citations: 3
|Lin F, Wang X, Jie Z, Hong X, Li X, Wang M, Yu Y. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):509-14. Doi: 10.1007/S11596-011-0481-5. Epub 2011 Aug 7. Inhibitory Effects Of Mir-146B-5P On Cell Migration And Invasion Of Pancreatic Cancer ...||PubMed ID 21823013|
|miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro||PubMed ID 26604788|
|MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma||PubMed ID 27292876|
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