|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-ErbB 4 Antibody|
|Description||Rabbit IgG polyclonal antibody for Receptor tyrosine-protein kinase erbB-4(ERBB4) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-ErbB 4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1881)|
|Replacement Item||This antibody may replace the following items: sc-283|sc-283-G|sc-284|sc-284-G|sc-31149|sc-31150|sc-31151|sc-33040|sc-514327|sc-514462|sc-515142|sc-53280|sc-71070|sc-71071|sc-7301|sc-8050|sc-81456|sc-81491 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 4(35-51aa KLSSLSDLEQQYRALRK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Receptor tyrosine-protein kinase erbB-4|
|Molecular Weight||146808 MW|
|Protein Function||Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis. .|
|Tissue Specificity||Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein . In response to NRG1 treatment, the activated receptor is internalized.|
|Alternative Names||Receptor tyrosine-protein kinase erbB-4;220.127.116.11;Proto-oncogene-like protein c-ErbB-4;Tyrosine kinase-type cell surface receptor HER4;p180erbB4;ERBB4 intracellular domain;4ICD;E4ICD;s80HER4;ERBB4;HER4;|
Background for Receptor tyrosine-protein kinase erbB-4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ErbB 4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ErbB 4 Antibody Images
Click the images to enlarge.
Lane 1: A549 Cell Lysate
Lane 2: HELA Cell Lysate
Recombinant Protein Detection Source: E.coli derived -recombinant human ERRB4, 40.6KD (162aa tag+M1-P200)
Lane 1: Recombinant Human ERRB4 Protein 10ng
Lane 2: Recombinant Human ERRB4 Protein 5ng
Lane 3: Recombinant Human ERRB4 Protein 2.5ng
Lane 4: Recombinant Human ERRB4 Protein 1.25ng
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: NEURO Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,