Anti-STAT5a Antibody

Stat5a antibody

Boster Bio Anti-STAT5a Antibody catalog # PA1840. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: PA1840
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-STAT5a Antibody

View all Stat5a Antibodies

SKU/Catalog Number

PA1840

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAT5a Antibody catalog # PA1840. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT5a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1840)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1840 is reactive to STAT5A in Human, Mouse, Rat

Applications

PA1840 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

90.647kDa

Background of Stat5a

STAT5 (Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al. (2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For STAT5A (Source: Uniprot.org, NCBI)

Gene Name

STAT5A

Full Name

Signal transducer and activator of transcription 5A

Weight

90.647kDa

Superfamily

transcription factor STAT family

Alternative Names

MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b STAT5A MGF, STAT5 signal transducer and activator of transcription 5A signal transducer and activator of transcription 5A|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on STAT5A, check out the STAT5A Infographic

STAT5A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STAT5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1840 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ocaranza P,Íñiguez G,Johnson MC,Cassorla F.Differential effects of androgens and estrogens over cellular GH sensitivity in HEPG2 cells.Growth Horm IGF Res.2021 May 4;57-58:101390.doi:10.1016/j.ghir.2021.101390.Epub ahead of print.PMID:33975196.
Species: Human
PA1840 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILTUION:1:2000

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...

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3 Customer Q&As for Anti-STAT5a Antibody

Question

See below the WB image, lot number and protocol we used for subcutaneous adipose tissue using anti-STAT5a antibody PA1840. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-31

Question

We are currently using anti-STAT5a antibody PA1840 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The anti-STAT5a antibody (PA1840) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-04

Question

I am interested in to test anti-STAT5a antibody PA1840 on rat subcutaneous adipose tissue for research purposes, then I may be interested in using anti-STAT5a antibody PA1840 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-20

Answer

The products we sell, including anti-STAT5a antibody PA1840, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-20

Order DetailsPrice
PA1840

100μg

$370
PA1840-10ug

10μg sample (liquid)

$99
PA1840-Biotin

100 μg Biotin conjugated

$570
PA1840-Cy3

100 μg Cy3 conjugated

$570
PA1840-Dylight488

100 μg Dylight488 conjugated

$570
PA1840-Dylight550

100 μg Dylight550 conjugated

$570
PA1840-Dylight594

100 μg Dylight594 conjugated

$570
PA1840-FITC

100 μg FITC conjugated

$570
PA1840-HRP

100 μg HRP conjugated

$570
PA1840-APC

100 μg APC conjugated

$670
PA1840-PE

100 μg PE conjugated

$670
PA1840-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1840
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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