Anti-STAT5a Antibody

Product Name

Anti-STAT5a Antibody

View all Stat5a Antibodies

SKU/Catalog Number

PA1840

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAT5a Antibody catalog # PA1840. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT5a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1840)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1840 is reactive to STAT5A in Human, Mouse, Rat

Applications

PA1840 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

90.647kDa

Background of Stat5a

STAT5 (Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al. (2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of STAT5A using anti-STAT5A antibody (PA1840).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified polyclonal antibody (Catalog # PA1840) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 95 kDa. The expected band size for STAT5A is at 91 kDa.

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Figure 2. Flow Cytometry analysis of U20S cells using anti-STAT5A antibody (PA1840).
Overlay histogram showing U20S cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5A Antibody (PA1840,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of Jurkat cells using anti-STAT5A antibody (PA1840).
Overlay histogram showing Jurkat cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5A Antibody (PA1840,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For STAT5A (Source: Uniprot.org, NCBI)

Alternative Names

MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b STAT5A MGF, STAT5 signal transducer and activator of transcription 5A signal transducer and activator of transcription 5A|epididymis secretory sperm binding protein

For more info on STAT5A, check out the STAT5A Infographic

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In this infographic, you will see the following information for STAT5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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