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Product Info Summary
| SKU: | M05470-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103
SKU/Catalog Number
M05470-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (Catalog # M05470-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM103
Isotype
Rabbit IgG
Immunogen
Mouse IgG
Cross-reactivity
This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross-reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
Reactive Species
M05470-2 is reactive to Igkc in Mouse
Calculated molecular weight
11.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05470-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.005ug/mL-0.2ug/mL
WB: 0.1ug/mL-0.5ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western blot of nonreduced(-) and reduced(+) mouse IgG1 and IgG1 (20ng/lane), using 0.2ug/mL of clone RM103. This antibody reacted to nonreduced IgG1 kappa (~150 kDa), and slightly react to reduced kappa light chain (~25 kDa)
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ELISA result showing specificity
ELISA of mouse immunoglobulins shows RM103 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA of mouse IgG1 Kappa. The plate was coated with different amounts of mouse IgG1 Kappa. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (M05470-2)
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