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Product Info Summary
| SKU: | M00857 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Mouse IL-27/P28 Monoclonal Antibody
SKU/Catalog Number
M00857
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Mouse IL-27/P28 Monoclonal Antibody (Catalog # M00857). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Mouse IL-27/P28 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00857)
Host
Rat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 3H12.F10 IgG2a kappa
Isotype
IgG2a kappa
Immunogen
Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Reactive Species
M00857 is reactive to IL27 in Mouse
Observed Molecular Weight
19 kDa
Calculated molecular weight
26.5 kDa
Background of IL27
Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00857 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000
Flow Cytometry: 1 ug / ml
WB: 1:1000
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.
Validation Images & Assay Conditions
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Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN gamma and 1ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1-10ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
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Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Boster's Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
Specific Publications For Anti-Mouse IL-27/P28 Monoclonal Antibody (M00857)
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Customer Q&As
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13 Customer Q&As for Anti-Mouse IL-27/P28 Monoclonal Antibody
Question
Can you help my question with product M00857, anti-Mouse IL-27/P28 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00857 anti-Mouse IL-27/P28 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-26
Question
Is there a BSA free version of anti-Mouse IL-27/P28 Monoclonal antibody M00857 available?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mouse IL-27/P28 Monoclonal antibody M00857 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use your anti-Mouse IL-27/P28 Monoclonal antibody for WB for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
As indicated on the product datasheet, M00857 anti-Mouse IL-27/P28 Monoclonal antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-09
Question
Does anti-Mouse IL-27/P28 Monoclonal antibody M00857 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
According to the expression profile of lung, IL27 is highly expressed in lung. So, it is likely that anti-Mouse IL-27/P28 Monoclonal antibody M00857 will work for WB with lung.
Boster Scientific Support
Answered: 2019-09-24
Question
My lab would like to test anti-Mouse IL-27/P28 Monoclonal antibody M00857 on mouse lung for research purposes, then I may be interested in using anti-Mouse IL-27/P28 Monoclonal antibody M00857 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The products we sell, including anti-Mouse IL-27/P28 Monoclonal antibody M00857, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-30
Question
See below the WB image, lot number and protocol we used for lung using anti-Mouse IL-27/P28 Monoclonal antibody M00857. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-Mouse IL-27/P28 Monoclonal antibody M00857 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The anti-Mouse IL-27/P28 Monoclonal antibody (M00857) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Mouse IL-27/P28 Monoclonal antibody M00857. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
Is a blocking peptide available for product anti-Mouse IL-27/P28 Monoclonal antibody (M00857)?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
We do provide the blocking peptide for product anti-Mouse IL-27/P28 Monoclonal antibody (M00857). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-25
Question
My colleagues were satisfied with the WB result of your anti-Mouse IL-27/P28 Monoclonal antibody. However we have been able to see positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL27 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
Based on literature, lung does express IL27. Generally IL27 expresses in secreted. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-25
Question
We have observed staining in mouse lung. Do you have any suggestions? Is anti-Mouse IL-27/P28 Monoclonal antibody supposed to stain lung positively?
F. Taylor
Verified customer
Asked: 2016-12-20
Answer
According to literature lung does express IL27. According to Uniprot.org, IL27 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-12-20
Question
I see that the anti-Mouse IL-27/P28 Monoclonal antibody M00857 works with WB, what is the protocol used to produce the result images on the product page?
Z. Krishna
Verified customer
Asked: 2016-05-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-05-05
Question
Would M00857 anti-Mouse IL-27/P28 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Roberts
Verified customer
Asked: 2014-03-20
Answer
As indicated on the product datasheet, M00857 anti-Mouse IL-27/P28 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-20
