Rabbit IgG polyclonal antibody for Interleukin-17A(IL17A) detection. Tested with WB, ELISA in Mouse. Cited in 2 publication(s).
Anti-IL17/IL17A Antibody Info At A Glance
|Product Name||Anti-IL17/IL17A Antibody
See all IL-17/IL-17A primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 17/IL17A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. IL 17/IL17A information: Molecular Weight: 17490 MW; Subcellular Localization: Secreted; Tissue Specificity: Restricted to a subset of activated T-cells.|
|Cite This Product||Anti-IL17/IL17A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1019)|
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|Anti-IL17/IL17A Antibody may replace the following items: sc 47877|sc 47876|sc 135215|sc 365715|sc 47875|sc 47877 X|sc 47876 X|sc 515041 X|sc 515041|sc 47875 X.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||E. coli-derived mouse IL-17 recombinant protein(Position: A26-A158).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Il17a (Source: Uniprot.org, NCBI)
|NCBI Gene Id||16171|
|Species Of This Entry||Mouse|
|Alternative Names||IL-17/IL-17A|CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A|
|Post Tranlational Modifications||Methylation; Phosphorylation; Demethylation; Acetylation|
|Gene Location||1|1 A4, on Chromasome 1, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il17a (Source: Uniprot.org, NCBI)
|Protein Function||Ligand for IL17RA (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (By similarity). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (By similarity).|
|Tissue Specificity||Restricted to a subset of activated T-cells.|
|Related Diseases||Inflammation; Autoimmune Reaction; Autoimmune Diseases; Arthritis; Asthma; Rheumatoid Arthritis; Colitis; Experimental Autoimmune Encephalomyelitis; Malignant Neoplasms; Encephalomyelitis; Intestinal Diseases; Infective Disorder; Pneumonia; Autoimmunity; Crohn Disease; Neoplasms; Inflammatory Bowel Diseases; Atherosclerosis; Ulcer; Psoriasis|
|Related Pathways||Pathogenesis; Secretion; Immune Response; Cytokine Production; Cell Differentiation; Methylation; Cell Proliferation; Inflammatory Response; Chemotaxis; Adaptive Immune Response; T Cell Differentiation; Sensitization; T Cell Proliferation; Cell Development; Cell Activation; T Cell Activation; Dna Methylation; Hypersensitivity; Demethylation; Cell Growth|
|Background||Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.|
|Scientific References||PMID: 8877732 by Kennedy J., et al. Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells.|
PMID: 8654948 by Yao Z., et al. Complete nucleotide sequence of the mouse CTLA8 gene.
Our Boster Quality Guarantee for Anti-IL17/IL17A Antibody covers its use in the following applications.ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Mouse IL-17 Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-17 antigen affinity purified polyclonal antibody (Catalog # RP1019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 2
|Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.||PubMed ID 25664038|
|Ma X, Wang L, Zhao H, Pang N, Zhang F, Jiang T, Liu X, Mamuti W, Wen H, Ding J. Mol Med Rep. 2014 Jul;10(1):236-40. Doi: 10.3892/Mmr.2014.2170. Epub 2014 Apr 24. Th17??cells Are Associated With The Th1/Th2?||PubMed ID 24789110|
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Note: sample size takes longer to process, average 5-7 days.
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