Anti-IL17/IL17A Antibody

IL-17/IL-17A antibody

Boster Bio Anti-IL17/IL17A Antibody catalog # RP1019. Tested in ELISA, WB applications. This antibody reacts with Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: RP1019
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL17/IL17A Antibody

View all IL-17/IL-17A Antibodies

SKU/Catalog Number

RP1019

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL17/IL17A Antibody catalog # RP1019. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL17/IL17A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1019)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse IL-17 recombinant protein (Position: A26-A158).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1019 is reactive to IL17A in Mouse

Applications

RP1019 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

17.504kDa

Background of IL-17/IL-17A

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For IL17A (Source: Uniprot.org, NCBI)

Gene Name

IL17A

Full Name

Interleukin-17A

Weight

17.504kDa

Superfamily

IL-17 family

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL17A, check out the IL17A Infographic

IL17A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1019 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma X, Wang L, Zhao H, Pang N, Zhang F, Jiang T, Liu X, Mamuti W, Wen H, Ding J. Mol Med Rep. 2014 Jul;10(1):236-40. Doi: 10.3892/Mmr.2014.2170. Epub 2014 Apr 24. Th17??cells Are Associated With The Th1/Th2?

Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.

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16 Customer Q&As for Anti-IL17/IL17A Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL17/IL17A antibody RP1019. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

Please see the WB image, lot number and protocol we used for t-cell using anti-IL17/IL17A antibody RP1019. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-27

Question

Our lab want to know about to test anti-IL17/IL17A antibody RP1019 on mouse t-cell for research purposes, then I may be interested in using anti-IL17/IL17A antibody RP1019 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

The products we sell, including anti-IL17/IL17A antibody RP1019, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-17

Question

I was wanting to use your anti-IL17/IL17A antibody for ELISA for mouse t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse t-cell identification?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

As indicated on the product datasheet, RP1019 anti-IL17/IL17A antibody has been validated for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-24

Question

Is a blocking peptide available for product anti-IL17/IL17A antibody (RP1019)?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

We do provide the blocking peptide for product anti-IL17/IL17A antibody (RP1019). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-28

Question

Would anti-IL17/IL17A antibody RP1019 work on monkey WB with t-cell?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

Our lab technicians have not tested anti-IL17/IL17A antibody RP1019 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IL17/IL17A antibody RP1019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-31

Question

Will anti-IL17/IL17A antibody RP1019 work for ELISA with t-cell?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

According to the expression profile of t-cell, IL17A is highly expressed in t-cell. So, it is likely that anti-IL17/IL17A antibody RP1019 will work for ELISA with t-cell.

Boster Scientific Support

Answered: 2019-08-01

Question

Is this RP1019 anti-IL17/IL17A antibody reactive to the isotypes of IL17A?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

The immunogen of RP1019 anti-IL17/IL17A antibody is E. coli-derived mouse IL-17 recombinant protein(Position: A26-A158). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-17

Question

Can you help my question with product RP1019, anti-IL17/IL17A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-03-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1019 anti-IL17/IL17A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-03-05

Question

Do you have a BSA free version of anti-IL17/IL17A antibody RP1019 available?

B. Jones

Verified customer

Asked: 2018-08-30

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL17/IL17A antibody RP1019 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-30

Question

I see that the anti-IL17/IL17A antibody RP1019 works with ELISA, what is the protocol used to produce the result images on the product page?

C. Huang

Verified customer

Asked: 2018-02-05

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-02-05

Question

We have been able to see staining in mouse t-cell. Any tips? Is anti-IL17/IL17A antibody supposed to stain t-cell positively?

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

According to literature t-cell does express IL17A. According to Uniprot.org, IL17A is expressed in palpebral conjunctiva, t-cell, among other tissues. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2017-06-02

Question

Does RP1019 anti-IL17/IL17A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Lewis

Verified customer

Asked: 2014-11-11

Answer

As indicated on the product datasheet, RP1019 anti-IL17/IL17A antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-11

Question

We are currently using anti-IL17/IL17A antibody RP1019 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?

R. Jones

Verified customer

Asked: 2014-03-28

Answer

The anti-IL17/IL17A antibody (RP1019) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-28

Question

We bought anti-IL17/IL17A antibody for ELISA on t-cell a few years ago. I am using mouse, and I plan to use the antibody for WB next. My question regards examining t-cell as well as palpebral conjunctiva in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Z. Lewis

Verified customer

Asked: 2013-06-28

Answer

I took a look at the website and datasheets of our anti-IL17/IL17A antibody and I see that RP1019 has been validated on mouse in both ELISA and WB. Thus RP1019 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-06-28

Question

We were happy with the WB result of your anti-IL17/IL17A antibody. However we have seen positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL17A supposed to be expressed?

S. Zhang

Verified customer

Asked: 2013-01-23

Answer

From literature, t-cell does express IL17A. Generally IL17A expresses in secreted. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2013-01-23

Order DetailsPrice
RP1019

100μg

$370
RP1019-10ug

10μg sample (liquid)

$99
RP1019-Biotin

100 μg Biotin conjugated

$570
RP1019-Cy3

100 μg Cy3 conjugated

$570
RP1019-Dylight488

100 μg Dylight488 conjugated

$570
RP1019-Dylight550

100 μg Dylight550 conjugated

$570
RP1019-Dylight594

100 μg Dylight594 conjugated

$570
RP1019-FITC

100 μg FITC conjugated

$570
RP1019-HRP

100 μg HRP conjugated

$570
RP1019-APC

100 μg APC conjugated

$670
RP1019-PE

100 μg PE conjugated

$670
RP1019-carrier-free

Carrier Free

$370

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