Anti-Myeloperoxidase/MPO Antibody

Boster Bio Anti-Myeloperoxidase/MPO Antibody catalog # PA1054. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: PA1054
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC-P, WB

Product Name

Anti-Myeloperoxidase/MPO Antibody

See all Myeloperoxidase/MPO products

SKU/Catalog Number

PA1054

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Myeloperoxidase/MPO Antibody catalog # PA1054. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myeloperoxidase/MPO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1054)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MPO (714-728aa KNNIFMSNSYPRDFV), different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1054 is reactive to MPO in Human, Mouse, Rat

Applications

PA1054 is guaranteed for IF, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunofluorescence|2μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For MPO (Source: Uniprot.Org, NCBI)

Uniprot ID

P05164

Gene ID

4353

Gene Name

MPO

Full Name

Myeloperoxidase

Weight

83.869kDa

Superfamily

peroxidase family

Alternative Names

EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MPO, check out the MPO Infographic

MPO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1054 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wei Gao,Qiushi Huang,Ting Qin et al.HSP60 regulates monosodium urate crystal-induced inflammation by activating the TLR4-NF- κ B-MyD88 signaling pathway and disrupting mitochondrial function,30 April 2020, PREPRINT(Version 1) available at Research Square
Species: Human,Mouse
PA1054 usage in article: APP:IF, SAMPLE:FOOT PAD TISSUE, DILUTION:NA

The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026

Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats

Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Wei CC, Kong YY, Li GQ, Guan YF, Wang P, Miao CY. Sci Rep. 2017 Apr 6;7(1):717. doi: 10.1038/s41598-017-00851-z. Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway.

Have you used Anti-Myeloperoxidase/MPO Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Myeloperoxidase/MPO Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

18 Customer Q&As for Anti-Myeloperoxidase/MPO Antibody

Question

Does anti-Myeloperoxidase/MPO antibody PA1054 work on canine IHC-P with bone marrow cell?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

Our lab technicians have not tested anti-Myeloperoxidase/MPO antibody PA1054 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Myeloperoxidase/MPO antibody PA1054 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine bone marrow cell in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-14

Question

Is a blocking peptide available for product anti-Myeloperoxidase/MPO antibody (PA1054)?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

We do provide the blocking peptide for product anti-Myeloperoxidase/MPO antibody (PA1054). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-27

Question

My question regarding product PA1054, anti-Myeloperoxidase/MPO antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1054 anti-Myeloperoxidase/MPO antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-20

Question

Our lab want to know about to test anti-Myeloperoxidase/MPO antibody PA1054 on human leukemia for research purposes, then I may be interested in using anti-Myeloperoxidase/MPO antibody PA1054 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

The products we sell, including anti-Myeloperoxidase/MPO antibody PA1054, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-30

Question

See attached the WB image, lot number and protocol we used for leukemia using anti-Myeloperoxidase/MPO antibody PA1054. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemia using anti-Myeloperoxidase/MPO antibody PA1054. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-10

Question

Can PA1054 is antibody be provided in a fluorescence conjugation format? If yes, what is the minimum size and the lead time?

Verified customer

Asked: 2019-07-05

Answer

We have to manufacture a new lot of Anti-Myeloperoxidase/MPO Antibody PA1054 for conjugation. The minimum size is 1mg and the lead time would be 3-4 months.

Boster Scientific Support

Answered: 2019-07-08

Question

Does PA1054 anti-Myeloperoxidase/MPO antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

It shows on the product datasheet, PA1054 anti-Myeloperoxidase/MPO antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-17

Question

Is this PA1054 anti-Myeloperoxidase/MPO antibody reactive to the isotypes of MPO?

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

The immunogen of PA1054 anti-Myeloperoxidase/MPO antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MPO (714-728aa KNNIFMSNSYPRDFV), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-22

Question

I would like using your anti-Myeloperoxidase/MPO antibody for oxidation-reduction process studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

We appreciate your inquiry. This PA1054 anti-Myeloperoxidase/MPO antibody is tested on rat brain tissue. It is guaranteed to work for IF, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-09

Question

Do you have a BSA free version of anti-Myeloperoxidase/MPO antibody PA1054 available?

Verified Customer

Verified customer

Asked: 2018-01-03

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Myeloperoxidase/MPO antibody PA1054 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-03

Question

We have been able to see staining in rat plasma. Are there any suggestions? Is anti-Myeloperoxidase/MPO antibody supposed to stain plasma positively?

D. Parker

Verified customer

Asked: 2017-12-12

Answer

From what I have seen in literature plasma does express MPO. From what I have seen in Uniprot.org, MPO is expressed in bone marrow cell, leukemia, leukocyte, plasma, saliva, liver, among other tissues. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2017-12-12

Question

We are currently using anti-Myeloperoxidase/MPO antibody PA1054 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

A. Gonzalez

Verified customer

Asked: 2017-10-26

Answer

The anti-Myeloperoxidase/MPO antibody (PA1054) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-26

Question

Our lab were well pleased with the WB result of your anti-Myeloperoxidase/MPO antibody. However we have seen positive staining in leukemia lysosome. using this antibody. Is that expected? Could you tell me where is MPO supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-16

Answer

From what I have seen in literature, leukemia does express MPO. Generally MPO expresses in lysosome. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2017-08-16

Question

Does anti-Myeloperoxidase/MPO antibody PA1054 work for WB with leukemia?

Verified Customer

Verified customer

Asked: 2017-06-06

Answer

According to the expression profile of leukemia, MPO is highly expressed in leukemia. So, it is likely that anti-Myeloperoxidase/MPO antibody PA1054 will work for WB with leukemia.

Boster Scientific Support

Answered: 2017-06-06

Question

We ordered your anti-Myeloperoxidase/MPO antibody for IF on bone marrow cell in the past. I am using mouse, and We are going to use the antibody for WB next. I am interested in examining bone marrow cell as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

J. Carter

Verified customer

Asked: 2015-04-03

Answer

I looked at the website and datasheets of our anti-Myeloperoxidase/MPO antibody and it seems that PA1054 has been validated on mouse in both IF and WB. Thus PA1054 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-04-03

Question

I was wanting to use your anti-Myeloperoxidase/MPO antibody for WB for human leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemia identification?

R. Brown

Verified customer

Asked: 2014-09-01

Answer

You can see on the product datasheet, PA1054 anti-Myeloperoxidase/MPO antibody has been tested for IF, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-09-01

Question

I see that the anti-Myeloperoxidase/MPO antibody PA1054 works with WB, what is the protocol used to produce the result images on the product page?

N. Parker

Verified customer

Asked: 2013-11-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-11-01

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PA1054
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.