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MPO antibody (PA1054) performs well in Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 33 Publications

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Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (PA1054).
Overlay histogram showing HL-60 cells stained with PA1054 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (PA1054, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Myeloperoxidase/MPO Antibody Picoband®

Name: Anti-Myeloperoxidase/MPO Antibody Picoband®
Brand: Boster Bio
SKU: PA1054
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

MPO antibody

33 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, WB

Tested Applications

MPO antibody was positively detected by WB in:

human HL-60 whole cell, rat thymus tissue, mouse spleen tissue, mouse thymus tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MPO antibody was positively detected by IHC in:

human tonsil tissue, mouse spleen tissue, rat lymphaden tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MPO antibody was positively detected by IF in:

human spleen tissue, human appendicitis tissue

MPO antibody was positively detected by FC in:

HL-60 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-Myeloperoxidase/MPO Antibody Picoband®
View all MPO Antibodies

Form

Lyophilized

Uniprot

P05164

Gene ID

4353

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MPO, different from the related mouse and rat sequences by one amino acid.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

60 kDa

Calculated Molecular Weight

83.9 kDa

Background of MPO

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Cite This Product

Anti-Myeloperoxidase/MPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1054)

Protocols

Western Blot protocol for MPO antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MPO antibody

Review Protocol

Immunofluorescence (IF) protocol for MPO antibody

Review Protocol

Flow Cytometry (FC) protocol for MPO antibody

Review Protocol

Publications

Mincle-Mediated Coxsackievirus-B3 Infection Induced Fulminant Myocarditis Via Trained Immunity

Published: 2026-06-10

Journal: Phenomics

Application: IHC

Impact Factor: 5.50

Species: Mouse

SKU	PA1054
Application	Immunohistochemistry
Sample	mouse skin tissue
Sample Processing Description	Male BALB/c mice aged 6–8 weeks were used. (1) Normal dorsal skin was collected from untreated mice. (2) Burn injury was induced on the dorsal skin using a burn device. After one week of housing, hair was removed with depilatory cream. Skin samples were collected from normal mice (dorsal skin) and burned mice (burned area), fixed in formalin for 72 hours, and embedded in paraffin for sectioning./td>
Other Reagents	Tris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAB Chromogen Kit
Primary Antibody	Myeloperoxidase/MPO Antibody Picoband®
Primary Incubation	1:100, overnight at 4 ℃
Secondary Antibody	Polymer Anti-Rabbit IgG–HRP Immunohistochemistry Kit
Detection	Imaging system:Leica DM2500
Results Summary	MPO (myeloperoxidase) is a lysosomal enzyme mainly present in neutrophils and monocytes/macrophages. Its core function is to generate reactive oxygen species, and therefore it plays a “double-edged sword” role in innate immune defense and inflammation-related diseases. MPO is present at very low levels in normal skin, but in burned skin, a large number of macrophages accumulate around the injured tissue, accompanied by a significant increase in MPO expression. Based on the immunohistochemical results, the staining is clear and the findings are consistent with expectations.

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Anti-Myeloperoxidase/MPO Antibody Picoband®

MPO antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Mincle-Mediated Coxsackievirus-B3 Infection Induced Fulminant Myocarditis Via Trained Immunity

Hormonal Treatment During Ex Vivo Lung Perfusion Ameliorates Brain Death Induced Inflammation

Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation

Inhibition of macrophage extracellular traps by Metrnl/IL-41 suppresses airway remodeling in asthma

Astragaloside IV Regulates the IRF7/NLRP3 Axis to Inhibit Neutrophil Extracellular Trap Formation and Alleviate Coxsackievirus B3-Induced Myocarditis

Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis

Ultrasmall PtIr Bimetallic Nanozyme Treats Myocardial Infarction via Ischemic/Inflammatory Cardiac Microenvironment Remodeling

Sex differences in kidney and lung status in an animal model of brain death

Granzyme K+CD8+ T cells interact with fibroblasts to promote neutrophilic inflammation in nasal polyps

APEX1 in intestinal epithelium triggers neutrophil infiltration and intestinal barrier damage in Ulcerative colitis

Spatiotemporal control of neutrophil fate to tune inflammation and repair for myocardial infarction therapy

Characterization and efficacy of C60 nano-photosensitive drugs in colorectal cancer treatment

Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome

17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats

Butylparaben disordered intestinal homeostasis in Chinese striped-necked turtles (Mauremys sinensis)

Biointerface mineralization generates ultraresistant gut microbes as oral biotherapeutics

Maresin-2 alleviates allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome, Th2 type immune response and oxidative stress

Hydroxysafflor yellow A protects against ulcerative colitis via suppressing TLR4/NF-κB signaling pathway

Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora

HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function

Matrix Metalloproteases-Mediated Cleavage on β-Dystroglycan May Play a Key Role in the Blood–Brain Barrier After Intracerebral Hemorrhage in Rats

DNase-1 Treatment Exerts Protective Effects in a Rat Model of Intestinal Ischemia-Reperfusion Injury.

miR-27a-3p protects against blood–brain barrier disruption and brain injury after intracerebral hemorrhage by targeting endothelial aquaporin-11

MPO Promoter Polymorphism rs2333227 Enhances Malignant Phenotypes of Colorectal Cancer by Altering the Binding Affinity of AP-2α

MicroRNA-126-3p attenuates blood-brain barrier disruption, cerebral edema and neuronal injury following intracerebral hemorrhage by regulating PIK3R2 and Akt

Interleukin-17A Aggravates Middle Ear Injury Induced by Streptococcus pneumoniae through the p38 Mitogen-Activated Protein Kinase Signaling Pathway

NAD replenishment with nicotinamide mononucleotide protects blood–brain barrier integrity and attenuates delayed tissue plasminogen activator-induced haemorrhagic transformation after cerebral ischaemia

Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway

The critical role of myeloperoxidase in Streptococcus pneumoniae clearance and tissue damage during mouse acute otitis media

The novel exercise-induced hormone irisin protects against neuronal injury via activation of the Akt and ERK1/2 signaling pathways and contributes to the neuroprotection of physical exercise in cerebral ischemia

Ligustilide ameliorates neuroinflammation and brain injury in focal cerebral ischemia/reperfusion rats: involvement of inhibition of TLR4/peroxiredoxin 6 signaling

Review

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1 Review For Anti-Myeloperoxidase/MPO Antibody Picoband®

Using Myeloperoxidase/MPO Antibody Picoband® (PA1054) in IHC, MPO showed low expression in normal mouse skin and was markedly increased in burned skin, with clear staining and expected results.

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