Click image to see more details
Product Info Summary
| SKU: | PB9049 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cathepsin G/CTSG Antibody Picoband®
SKU/Catalog Number
PB9049
PB0021 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin G/CTSG Antibody Picoband® catalog # PB9049. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin G/CTSG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9049)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9049 is reactive to CTSG in Human, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
28.8 kDa
Background of CTSG
Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSG gene. This gene is mapped to 14q12, human Cathepsin G gene was expressed in early myeloid precursors in a manner coordinate with the expression of the endogenous murine gene in the bone marrow and spleen. The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9049 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human THP-1 whole cell, rat liver tissue
IHC: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (PB9049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29 kDa. The expected band size for Cathepsin G is at 29 kDa.
Click image to see more details
Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (PB9049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29 kDa. The expected band size for Cathepsin G is at 29 kDa.
Click image to see more details
IHC analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049).
Cathepsin G was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin G Antibody (PB9049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Cathepsin G/CTSG Antibody Picoband® (PB9049)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cathepsin G/CTSG Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Cathepsin G/CTSG Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Cathepsin G/CTSG Antibody Picoband®
Question
Will anti-Cathepsin G/CTSG antibody PB9049 work for WB with monocyte?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
According to the expression profile of monocyte, CTSG is highly expressed in monocyte. So, it is likely that anti-Cathepsin G/CTSG antibody PB9049 will work for WB with monocyte.
Boster Scientific Support
Answered: 2020-03-04
Question
Will anti-Cathepsin G/CTSG antibody PB9049 work on monkey WB with neutrophil?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not validated anti-Cathepsin G/CTSG antibody PB9049 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Cathepsin G/CTSG antibody PB9049 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey neutrophil in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
Please see the WB image, lot number and protocol we used for monocyte using anti-Cathepsin G/CTSG antibody PB9049. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
Would PB9049 anti-Cathepsin G/CTSG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
It shows on the product datasheet, PB9049 anti-Cathepsin G/CTSG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-16
Question
Can you help my question with product PB9049, anti-Cathepsin G/CTSG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Zhao
Verified customer
Asked: 2016-09-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9049 anti-Cathepsin G/CTSG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-02
Question
We are currently using anti-Cathepsin G/CTSG antibody PB9049 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
J. Jackson
Verified customer
Asked: 2016-04-25
Answer
The anti-Cathepsin G/CTSG antibody (PB9049) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-25
Question
Is this PB9049 anti-Cathepsin G/CTSG antibody reactive to the isotypes of CTSG?
W. Dhar
Verified customer
Asked: 2014-12-19
Answer
The immunogen of PB9049 anti-Cathepsin G/CTSG antibody is E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-19
