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Product Info Summary
|Reactive Species:||Human, Rat|
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Anti-Cathepsin G/CTSG Antibody Picoband™
Boster Bio Anti-Cathepsin G/CTSG Antibody Picoband™ catalog # PB9049. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin G/CTSG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9049)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9049 is reactive to CTSG in Human, Rat
PB9049 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (A04887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (Catalog # A04887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 38 kDa. The expected band size for Cathepsin G is at 29 kDa.
Gene/Protein Information For CTSG (Source: Uniprot.Org, NCBI)
peptidase S1 family
cathepsin G; CathepsinG; CGCATG; EC 3.4.21; EC 184.108.40.206; MGC23078 CTSG CATG, CG cathepsin G cathepsin G*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CTSG, check out the CTSG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9049
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