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MPO antibody (PB9057) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (PB9057).
Overlay histogram showing HL-60 cells stained with PB9057 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (PB9057, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HL-60 cells stained with PB9057 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (PB9057, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Myeloperoxidase/MPO Antibody Picoband®
MPO antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
MPO antibody was positively detected by WB in:
human HL-60 whole cell, human A431 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MPO antibody was positively detected by IHC in:
human spleen tissue, human lung adenocarcinoma tissue, human endometrial adenocarcinoma tissue, human endometrial cancer tissue, mouse liver tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MPO antibody was positively detected by FC in:
HL-60 cell
MPO antibody was positively detected by IF in:
human endometrial cancer tissue, human stomach cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Myeloperoxidase/MPO Antibody Picoband®
View all MPO Antibodies
View all MPO Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745). Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60-65 kDa, 80-90 kDa
Calculated Molecular Weight
83.9 kDa
Background of MPO
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
Cite This Product
Anti-Myeloperoxidase/MPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9057)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Rat
90%
High Homology
Dog
90%
High Homology
Mouse
89%
Moderate Homology
Sheep
88%
Moderate Homology
Rabbit
87%
Moderate Homology
Cow
86%
Moderate Homology
Zebrafish
57%
Low homology
Drosophila
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MPO antibody
Immunohistochemistry (IHC) protocol for MPO antibody
Flow Cytometry (FC) protocol for MPO antibody
Immunofluorescence (IF) protocol for MPO antibody
Publications
Application:
Species:
Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study
Published: 2026-03-23
Journal: INTERNATIONAL DENTAL JOURNAL
Application: IHC
Impact Factor: 3.70
Species: Human
PMID: 41875836
Establishment of a novel mouse peritoneal dialysis-associated peritoneal injury model
Published: 2022-03-30
Journal: Clinical and Experimental Nephrology
Application: IHC
Impact Factor: 2.62
Species: Mouse
PMID: 35353282
Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue
Published: 2020-09-15
Journal: American Journal of Translational Research
Application: IHC
Impact Factor: 3.38
Species: Mouse
PMID: 33042410
From nutrition to medicine: Assessing hemorrhoid healing activity of Solanum melongena L. via in vivo experimental models and its major chemicals
Published: 2020-07-18
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 3.69
Species: Rat
PMID: 32687958
Salivary changes in oxidative stress related to inflammation in oral and gastrointestinal diseases
Published: 2020-07-09
Journal: ORAL DISEASES
Application: WB
Impact Factor: 2.61
Species: Human
PMID: 32643850
The neuroprotective effects and probable mechanisms of Ligustilide and its degradative products on intracerebral hemorrhage in mice
Published: 2018-07-31
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 3.12
Species: Mouse
PMID: 30075428
Resolvin D2 Limits Secondary Tissue Necrosis After Burn Wounds in Rats
Published: 2018-01-16
Journal: Journal of Burn Care & Research
Application: IHC
Impact Factor: 1.92
Species: Rat
PMID: 28877131
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo
Published: 2018-01-11
Journal: MOLECULAR IMMUNOLOGY
Application: IHC
Impact Factor: 3.19
Species: Mouse
PMID: 29331803
Epithelial disruptions, but not immune cell invasion, induced secretory dysfunction following innate immune activation in a novel model of acute salivary gland injury
Published: 2017-11-21
Journal: JOURNAL OF ORAL PATHOLOGY & MEDICINE
Application: IHC
Impact Factor: 2.04
Species: Mouse
PMID: 29160910
Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats
Published: 2017-07-14
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 3.37
Species: Rat
PMID: 28765694
Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
Published: 2017-04-06
Journal: Scientific Reports
Impact Factor: 4.26
PMID: 28386082
GHIP in Streptococcus pneumoniae is involved in antibacterial resistance and elicits a strong innate immune response through TLR2 and JNK/p38MAPK
Published: 2014-07-02
Journal: FEBS Journal
Application: IHC
Impact Factor: 3.99
Species: Mouse
PMID: 24989111
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Product Promise
We promise Anti-Myeloperoxidase/MPO Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9057
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026