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Product Info Summary
| SKU: | A01871-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MPYS TMEM173 Antibody
SKU/Catalog Number
A01871-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MPYS TMEM173 Antibody (Catalog # A01871-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MPYS antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MPYS TMEM173 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01871-1)
Host
Rabbit
Contents
MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-MPYS antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260-310 of MPYS.
Reactive Species
A01871-1 is reactive to TMEM173 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.2 kDa
Background of TMEM173
MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01871-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Recommend Dilution
WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: MPYS A01871-1 (1 μg/mL), MPYS 64-236 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human THP-1 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: MPYS A01871-1 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: MPYS A01871-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse A-20 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: MPYS A01871-1 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunocytochemistry Validation of MPYS in Mouse A20 Cells
Immunocytochemical analysis of A20 cells using anti-MPYS antibody (A01871-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of MPYS in Moue A20 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with A01871-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-MPYS TMEM173 Antibody (A01871-1)
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