Anti-Mre11 Antibody

Mre11 antibody

Boster Bio Anti-Mre11 Antibody (Catalog # A00731). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: A00731
Size: 100ug
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-Mre11 Antibody

View all Mre11 Antibodies

SKU/Catalog Number

A00731

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Mre11 Antibody (Catalog # A00731). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Mre11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00731)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 68-706 of mouse Mre11 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00731 is reactive to Mre11 in Mouse

Applications

A00731 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

80.223kDa

Background of Mre11

Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them close. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres, the MRN complex may modulate t-loop formulation. Anti-Mre11 Antibody is useful for researcher interested in DNA Damage and Repair and Nuclear Signaling Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For Mre11 (Source: Uniprot.org, NCBI)

Gene Name

Mre11

Full Name

Double-strand break repair protein MRE11

Weight

80.223kDa

Superfamily

MRE11/RAD32 family

Alternative Names

ATLDmeiotic recombination (S. cerevisiae) 11 homolog A; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); Mre11; MRE11A; MRE11MRE11B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Mre11, check out the Mre11 Infographic

Mre11 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Mre11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Mre11 Antibody

Question

Will A00731 anti-Mre11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

You can see on the product datasheet, A00731 anti-Mre11 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-09

Question

Do you have a BSA free version of anti-Mre11 antibody A00731 available?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mre11 antibody A00731 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-09

Question

Can you help my question with product A00731, anti-Mre11 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00731 anti-Mre11 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-11

Question

I see that the anti-Mre11 antibody A00731 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-09

Question

Is a blocking peptide available for product anti-Mre11 antibody (A00731)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

We do provide the blocking peptide for product anti-Mre11 antibody (A00731). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-24

Question

Is this A00731 anti-Mre11 antibody reactive to the isotypes of MRE11A?

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

The immunogen of A00731 anti-Mre11 antibody is A recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-14

Order DetailsPrice
A00731

100 μg

$599

Free Secondary Antibody

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A00731
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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