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Product Info Summary
| SKU: | M12749 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-MRPS7 Mouse Monoclonal Antibody [Clone ID: OTI5E11]
SKU/Catalog Number
M12749
Size
100 µl
Description
Boster Bio MRPS7 mouse monoclonal antibody, clone OTI5E11 (formerly 5E11). Catalog# M12749. Tested in WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-MRPS7 Mouse Monoclonal Antibody [Clone ID: OTI5E11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12749)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5E11
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 38-242 of human MRPS7 (NP_057055) produced in E.coli.
Reactive Species
M12749 is reactive to MRPS7 in Human
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12749 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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Western blot analysis of A549 cell lysate (35ug) by using anti-MRPS7 monoclonal antibody. Dilution: 1:500
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS7.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MRPS7-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MRPS7 monoclonal antibody M12749 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-MRPS7 Mouse Monoclonal Antibody [Clone ID: OTI5E11] (M12749)
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