Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF11B in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 5pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0480
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Product Name

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™

See all Osteoprotegerin/TNFRSF11B products

SKU/Catalog Number

EK0480

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF11B in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0480)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-L401

Sensitivity

<5 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0480 is reactive to TNFRSF11B in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK0480 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0480-CAP Anti-Human TNFRSF11B Pre-coated 96-well strip microplate 1
EK0480-ST Human TNFRSF11B Standard 2 vials, 10 ng/tube
EK0480-DA Human TNFRSF11B Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.5375750150030006000
O.D.0.0020.0810.1630.2820.5500.8411.5062.019

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Osteoprotegerin/TNFRSF11B, Dilution ratio of 1:1, concentration in serum is around 750pg/ml.

Some articles we found to cite concentrations of Osteoprotegerin/TNFRSF11B in samples: 24845712 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13265026961306922678
Standard deviation10.4237.7113.2311.5751.2136.57
CV (%)7.9%5.8%4.2%8.9%7.4%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Osteoprotegerin/TNFRSF11B in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11321421371331363.932.8%
Sample 265062565158562726.784.2%
Sample 326963024265728252800143.235.1%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF11B (Source: Uniprot.Org, NCBI)

Uniprot ID

O00300

Gene ID

4982

Gene Name

TNFRSF11B

Full Name

Tumor necrosis factor receptor superfamily member 11B

Weight

46.026kDa

Alternative Names

OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b TNFRSF11B OCIF, OPG, PDB5, TR1 TNF receptor superfamily member 11b tumor necrosis factor receptor superfamily member 11B|osteoclastogenesis inhibitory factor|osteoprotegerin|tumor necrosis factor receptor superfamily, member 11b

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF11B, check out the TNFRSF11B Infographic

TNFRSF11B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF11B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0480 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Bozkurt Doğan Ş,Öngöz Dede F,Ballı U,Sertoğlu E.Emerging roles of Interleukin-34 together with receptor activator of nuclear factor-kB ligand and osteoprotegerin levels in periodontal disease.Cytokine.2021 May 22;144:155584.doi:10.1016/j.cyto.2021.155584.Epub ahead of print.PMID:34034144.
Species: Human
EK0480 usage in article: APP:ELISA, SAMPLE:GCF TISSUE, DILUTION:NA

Park J,Jung MJ,Chung WY.The downregulation of IGFBP3 by TGF-β signaling in oral cancer contributes to the osteoclast differentiation. Biochem Biophys Res Commun. 2020 Nov 27:S0006-291X(20)32118-5.doi: 10.1016/j.bbrc.2020.11.073.Epub ahead of print.PMID:33
Species: Mouse,Human
EK0480 usage in article: APP:ELISA, SAMPLE:HFOB1.19 OSTEOBLASTIC CELLS, DILUTION:NA

Severe vitamin D-deficiency and increased bone turnover in patients with hepatitis B from northeastern China

Premature atherosclerosis in children with beta-thalassemia major: New diagnostic marker

Loss of RUNX3 expression inhibits bone invasion of oral squamous cell carcinoma

Higher Urinary Levels of 8-Hydroxy-2?-deoxyguanosine Are Associated with a Worse RANKL/OPG Ratio in Postmenopausal Women with Osteopenia

Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis

Mamolini, E., Cervellati, C., Greco, P., Carrieri, A., Massari, L., Crivellari, I.,…& Bonaccorsi, G. (2017). VDR, RANKL and OPG polymorphisms as possible predisposing cofactors of postmenopausal osteoporosis: explorative study in Italian populatio...

Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.

Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...

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8 Customer Q&As for Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™

Question

Q: can you recommend the dilution ratio of serum samples for detection of Osteoprotegerin in Human saliva? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-12-13

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Osteoprotegerin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-12-13

Question

Q: is it okay to use citrate plasma as samples in Human Osteoprotegerin Picokine® ELISA Kit (Catalog # EK0480)?

Verified Customer

Verified customer

Asked: 2020-11-14

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Osteoprotegerin causing degradation of its protein structure. Osteoprotegerin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Osteoprotegerin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-14

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Osteoprotegerin using the EK0480 Human Osteoprotegerin Picokine® ELISA Kit?

B. Miller

Verified customer

Asked: 2019-10-18

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-10-18

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-02-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-02-28

Question

Q: What is the optimal O.D. value for Osteoprotegerin ELISA kit? I performed your Osteoprotegerin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Osteoprotegerin even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Osteoprotegerin ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of Osteoprotegerin that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2018-10-03

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-05-26

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-05-26

Question

Q: if the enzyme conjugated Osteoprotegerin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Osteoprotegerin antigen?

Verified Customer

Verified customer

Asked: 2016-02-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-02-25

Question

Q: Can Osteoprotegerin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2014-02-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-02-11

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