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Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF11B in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.. Sensitivity: 5pg/ml. Cited in 10 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.|
|Sample Volume:||100μl per well|
|Assay Range:||93.7 pg/ml - 6,000 pg/ml|
|Product Name||Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™
See more Osteoprotegerin/TNFRSF11B products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF11B in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.. Sensitivity: 5pg/ml.|
|Cite This Product||Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0480)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.|
|Assay Range||93.7 pg/ml - 6,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: E22-L401|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||4982|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor receptor superfamily member 11B|
|Alternative Names||Osteoprotegerin/TNFRSF11B|OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TNFRSF11B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0480-CAP||Anti-Human TNFRSF11B Pre-coated 96-well strip microplate||1|
|EK0480-ST||Human TNFRSF11B Standard||2 vials, 10 ng/tube|
|EK0480-DA||Human TNFRSF11B Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0480 Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Bozkurt Doğan Ş,Öngöz Dede F,Ballı U,Sertoğlu E.Emerging roles of Interleukin-34 together with receptor activator of nuclear factor-kB ligand and osteoprotegerin levels in periodontal disease.Cytokine.2021 May 22;144:155584.doi:10.1016/j.cyto.2021.155584.Epub ahead of print.PMID:34034144.
EK0480 usage in article: APP:ELISA, SAMPLE:GCF TISSUE, DILUTION:NA
|PubMed ID 34034144|
|Park J,Jung MJ,Chung WY.The downregulation of IGFBP3 by TGF-β signaling in oral cancer contributes to the osteoclast differentiation. Biochem Biophys Res Commun. 2020 Nov 27:S0006-291X(20)32118-5.doi: 10.1016/j.bbrc.2020.11.073.Epub ahead of print.PMID:33
EK0480 usage in article: APP:ELISA, SAMPLE:HFOB1.19 OSTEOBLASTIC CELLS, DILUTION:NA
|PubMed ID 33256984|
|Severe vitamin D-deficiency and increased bone turnover in patients with hepatitis B from northeastern China||PubMed ID 24073717|
|Premature atherosclerosis in children with beta-thalassemia major: New diagnostic marker||PubMed ID 28279156|
|Loss of RUNX3 expression inhibits bone invasion of oral squamous cell carcinoma||PubMed ID 28030842|
|Higher Urinary Levels of 8-Hydroxy-2?-deoxyguanosine Are Associated with a Worse RANKL/OPG Ratio in Postmenopausal Women with Osteopenia||PubMed ID 26635910|
|Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis||PubMed ID 23025387|
|Mamolini, E., Cervellati, C., Greco, P., Carrieri, A., Massari, L., Crivellari, I.,…& Bonaccorsi, G. (2017). VDR, RANKL and OPG polymorphisms as possible predisposing cofactors of postmenopausal osteoporosis: explorative study in Italian populatio...||PubMed ID 28488893|
|Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.||PubMed ID 22057136|
|Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...||PubMed ID 22818895|
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Comprehensive info on TNFRSF11B, beautifully presented.Open Infographic
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