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- Table of Contents
MSH2 antibody (M00140-5) performs well in IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5).
MSH2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
MSH2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Anti-MSH2 Antibody Picoband® (monoclonal, 6B4F7)
MSH2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, IHC, ICC, WB |
Tested Applications
MSH2 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human A549 whole cell, human COLO-320 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MSH2 antibody was positively detected by IHC in:
human laryngeal squamous cell carcinomas tissue, human liver cancer tissue, human serous adenocarcinoma of ovary tissue, human invasive urothelial carcinoma tissue, human colorectal adenocarcinoma tissue, human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MSH2 antibody was positively detected by ICC/IF in:
Caco-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MSH2 Antibody Picoband® (monoclonal, 6B4F7)
View all MSH2 Antibodies
View all MSH2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6B4F7
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human MSH2 recombinant protein (Position: Q337-N583). Human MSH2 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat MSH2, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
105 kDa
Calculated Molecular Weight
104.7 kDa
Background of MSH2
DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms aheterodimer with MSH6 to make the human MutSα mismatch repair complex. It also dimerizes with MSH3 to form the MutSβ DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.
Cite This Product
Anti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00140-5)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
98%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Rat
94%
High Homology
Mouse
94%
High Homology
Zebrafish
75%
Moderate Homology
Rabbit
69%
Low homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MSH2 antibody
Immunohistochemistry (IHC) protocol for MSH2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MSH2 antibody
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Product Promise
We promise Anti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00140-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026