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Product Info Summary
| SKU: | A03994-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-JMJD1A KDM3A Antibody
SKU/Catalog Number
A03994-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD1A KDM3A Antibody (Catalog # A03994-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
JMJD1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD1A KDM3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03994-1)
Host
Rabbit
Contents
JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-JMJD1A antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A.
Cross-reactivity
This antibody will not cross-react with JMJD1B or JMJD1C.
Reactive Species
A03994-1 is reactive to KDM3A in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
147.3 kDa
Background of KDM3A
The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03994-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: JMJD1A, A03994-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human HepG2 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: JMJD1A, A03994-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: JMJD1A, A03994-1 (2 μg/mL), JMJD1A, 5367 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (A03994-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of JMJD1A in Rat Liver Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with A03994-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-JMJD1A KDM3A Antibody (A03994-1)
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