Anti-mTOR/MTOR Antibody Picoband™

TOR/mTOR antibody

Boster Bio Anti-mTOR/MTOR Antibody Picoband™ catalog # A00003-2. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey. Cited in 3 publication(s).

Product Info Summary

SKU: A00003-2
Size: 100μg/vial
Reactive Species: Human, Monkey
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-mTOR/MTOR Antibody Picoband™

View all TOR/mTOR Antibodies

SKU/Catalog Number

A00003-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-mTOR/MTOR Antibody Picoband™ catalog # A00003-2. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-mTOR/MTOR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00003-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00003-2 is reactive to MTOR in Human, Monkey

Applications

A00003-2 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Background of TOR/mTOR

The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MTOR (Source: Uniprot.Org, NCBI)

Uniprot ID

P42345

Gene ID

2475

Gene Name

MTOR

Full Name

Serine/threonine-protein kinase mTOR

Weight

288.892kDa

Superfamily

PI3/PI4-kinase family

Alternative Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/thr MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MTOR, check out the MTOR Infographic

MTOR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MTOR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00003-2 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
A00003-2 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Correlation between autophagy of osteoblasts and oxidative stress of osteoporosis rats

Rapamycin provides anti%u2011epileptogenic effect in a rat model of post%u2011traumatic epilepsy via deactivation of mTOR signaling pathway

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16 Customer Q&As for Anti-mTOR/MTOR Antibody Picoband™

Question

We are currently using anti-mTOR/MTOR antibody A00003-2 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

The anti-mTOR/MTOR antibody (A00003-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-18

Question

Is a blocking peptide available for product anti-mTOR/MTOR antibody (A00003-2)?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

We do provide the blocking peptide for product anti-mTOR/MTOR antibody (A00003-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-13

Question

Does A00003-2 anti-mTOR/MTOR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

It shows on the product datasheet, A00003-2 anti-mTOR/MTOR antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-02

Question

My question regards using your anti-mTOR/MTOR antibody for t-helper 1 cell lineage commitment studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Thanks for your inquiry. This A00003-2 anti-mTOR/MTOR antibody is tested on human hela, k562 whole cell lysates, hela whole cell lysates, u2os whole cell lysates, a549 whole cell lysates. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-25

Question

you antibody to test anti-mTOR/MTOR antibody A00003-2 on rat brain for research purposes, then I may be interested in using anti-mTOR/MTOR antibody A00003-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

The products we sell, including anti-mTOR/MTOR antibody A00003-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-28

Question

We purchased anti-mTOR/MTOR antibody for IHC-P on b-cell last year. I am using human, and We intend to use the antibody for ELISA next. We want examining b-cell as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

I have checked the website and datasheets of our anti-mTOR/MTOR antibody and it appears that A00003-2 has been validated on human in both IHC-P and ELISA. Thus A00003-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-17

Question

Can you help my question with product A00003-2, anti-mTOR/MTOR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Gonzalez

Verified customer

Asked: 2019-09-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00003-2 anti-mTOR/MTOR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-19

Question

We have observed staining in rat cerebellum. What should we do? Is anti-mTOR/MTOR antibody supposed to stain cerebellum positively?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

From what I have seen in literature cerebellum does express MTOR. From what I have seen in Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-06-21

Question

I see that the anti-mTOR/MTOR antibody A00003-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-05

Question

I was wanting to use your anti-mTOR/MTOR antibody for ELISA for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

As indicated on the product datasheet, A00003-2 anti-mTOR/MTOR antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-11

Question

Is this A00003-2 anti-mTOR/MTOR antibody reactive to the isotypes of MTOR?

Verified Customer

Verified customer

Asked: 2018-01-10

Answer

The immunogen of A00003-2 anti-mTOR/MTOR antibody is E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-10

Question

Is there a BSA free version of anti-mTOR/MTOR antibody A00003-2 available?

M. Wu

Verified customer

Asked: 2017-11-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR/MTOR antibody A00003-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-22

Question

I have attached the WB image, lot number and protocol we used for brain using anti-mTOR/MTOR antibody A00003-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-09-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-mTOR/MTOR antibody A00003-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-27

Question

Would anti-mTOR/MTOR antibody A00003-2 work for ELISA with brain?

N. Mangal

Verified customer

Asked: 2016-10-26

Answer

According to the expression profile of brain, MTOR is highly expressed in brain. So, it is likely that anti-mTOR/MTOR antibody A00003-2 will work for ELISA with brain.

Boster Scientific Support

Answered: 2016-10-26

Question

My team were content with the WB result of your anti-mTOR/MTOR antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?

C. Thomas

Verified customer

Asked: 2013-10-11

Answer

From literature, cervix carcinoma erythroleukemia does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2013-10-11

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00003-2

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00003-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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