Click image to see more details
Product Info Summary
| SKU: | A00003-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-mTOR/MTOR Antibody Picoband®
SKU/Catalog Number
A00003-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-mTOR/MTOR Antibody Picoband® catalog # A00003-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mTOR/MTOR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00003-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00003-2 is reactive to MTOR in Human, Monkey
Observed Molecular Weight
289 kDa
Calculated molecular weight
288.9 kDa
Background of MTOR
The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00003-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human K562 whole cell, human HEK293 whole cell, human Hela whole cell, monkey COS-7 whole cell, human A431 whole cell, human Jurkat whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of MTOR using anti-MTOR antibody (A00003-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human Jurkat whole cell ltsates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
Click image to see more details
IHC analysis of MTOR using anti-MTOR antibody (A00003-2).
MTOR was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-mTOR/MTOR Antibody Picoband® (A00003-2)
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Customer Q&As
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16 Customer Q&As for Anti-mTOR/MTOR Antibody Picoband®
Question
We are currently using anti-mTOR/MTOR antibody A00003-2 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The anti-mTOR/MTOR antibody (A00003-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-18
Question
Is a blocking peptide available for product anti-mTOR/MTOR antibody (A00003-2)?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We do provide the blocking peptide for product anti-mTOR/MTOR antibody (A00003-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-13
Question
Does A00003-2 anti-mTOR/MTOR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
It shows on the product datasheet, A00003-2 anti-mTOR/MTOR antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-02
Question
My question regards using your anti-mTOR/MTOR antibody for t-helper 1 cell lineage commitment studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thanks for your inquiry. This A00003-2 anti-mTOR/MTOR antibody is tested on human hela, k562 whole cell lysates, hela whole cell lysates, u2os whole cell lysates, a549 whole cell lysates. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-25
Question
you antibody to test anti-mTOR/MTOR antibody A00003-2 on rat brain for research purposes, then I may be interested in using anti-mTOR/MTOR antibody A00003-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The products we sell, including anti-mTOR/MTOR antibody A00003-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-28
Question
We purchased anti-mTOR/MTOR antibody for IHC-P on b-cell last year. I am using human, and We intend to use the antibody for ELISA next. We want examining b-cell as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
I have checked the website and datasheets of our anti-mTOR/MTOR antibody and it appears that A00003-2 has been validated on human in both IHC-P and ELISA. Thus A00003-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-17
Question
Can you help my question with product A00003-2, anti-mTOR/MTOR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Gonzalez
Verified customer
Asked: 2019-09-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00003-2 anti-mTOR/MTOR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
We have observed staining in rat cerebellum. What should we do? Is anti-mTOR/MTOR antibody supposed to stain cerebellum positively?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
From what I have seen in literature cerebellum does express MTOR. From what I have seen in Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-21
Question
I see that the anti-mTOR/MTOR antibody A00003-2 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-05
Question
I was wanting to use your anti-mTOR/MTOR antibody for ELISA for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
As indicated on the product datasheet, A00003-2 anti-mTOR/MTOR antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-11
Question
Is this A00003-2 anti-mTOR/MTOR antibody reactive to the isotypes of MTOR?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The immunogen of A00003-2 anti-mTOR/MTOR antibody is E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-10
Question
Is there a BSA free version of anti-mTOR/MTOR antibody A00003-2 available?
M. Wu
Verified customer
Asked: 2017-11-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR/MTOR antibody A00003-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-22
Question
I have attached the WB image, lot number and protocol we used for brain using anti-mTOR/MTOR antibody A00003-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-mTOR/MTOR antibody A00003-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-27
Question
Would anti-mTOR/MTOR antibody A00003-2 work for ELISA with brain?
N. Mangal
Verified customer
Asked: 2016-10-26
Answer
According to the expression profile of brain, MTOR is highly expressed in brain. So, it is likely that anti-mTOR/MTOR antibody A00003-2 will work for ELISA with brain.
Boster Scientific Support
Answered: 2016-10-26
Question
My team were content with the WB result of your anti-mTOR/MTOR antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?
C. Thomas
Verified customer
Asked: 2013-10-11
Answer
From literature, cervix carcinoma erythroleukemia does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2013-10-11
