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- Table of Contents
MTOR antibody (A00003-2) performs well in ELISA, IHC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of MTOR using anti-MTOR antibody (A00003-2).
MTOR was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
MTOR was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-mTOR/MTOR Antibody Picoband®
MTOR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | ELISA, IHC, WB |
Tested Applications
MTOR antibody was positively detected by WB in:
human K562 whole cell, human HEK293 whole cell, human Hela whole cell, monkey COS-7 whole cell, human A431 whole cell, human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MTOR antibody was positively detected by IHC in:
human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-mTOR/MTOR Antibody Picoband®
View all MTOR Antibodies
View all MTOR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
289 kDa
Calculated Molecular Weight
288.9 kDa
Background of MTOR
The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Cite This Product
Anti-mTOR/MTOR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00003-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Monkey
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Zebrafish
96%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MTOR antibody
Immunohistochemistry (IHC) protocol for MTOR antibody
Publications
Application:
Species:
Momordicine I Mediates Lipid-Lowering Effects of Momordica Charantia L. Triterpenoid Saponins via TFEB-Driven Lysosomal Biogenesis
Published: 2026-02-13
Journal: JOURNAL OF FOOD SCIENCE
Application: WB
Impact Factor: 3.40
Species: Human
PMID: 41684281
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway
Published: 2021-02-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 2.95
Species: Rat
PMID: 33649809
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway
Published: 2021-01-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 33537831
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells
Published: 2021-01-26
Journal: ENVIRONMENTAL POLLUTION
Application: WB
Impact Factor: 8.07
Species: Mouse
PMID: 33588191
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro
Published: 2019-07-05
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.59
Species: Human
PMID: 31283929
Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts
Published: 2017-04-05
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 1.69
Species: Human
PMID: 28393182
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Product Promise
We promise Anti-mTOR/MTOR Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00003-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026