Product Info Summary
| SKU: | A09710 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Cybr CYTIP Antibody
SKU/Catalog Number
A09710
Size
100uL
Form
Lyophilized
Description
Boster Bio Anti-Cybr CYTIP Antibody (Catalog # A09710). Tested in ELISA, IP, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store PSCDBP antibody / Anti-Cybr at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-Cybr CYTIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09710)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Reactive Species
A09710 is reactive to CYTIP in Human, Mouse, Rat
Calculated molecular weight
40.1 kDa
Background of CYTIP
Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09710 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:1,000 - 1:10,000
This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting after IP in the appropriate cell lysate or extract. To date, this antibody has not shown direct detection of endogenous or over-expressed Cybr protein, but is capable of immunoprecipitating the antigen from lysates. It is possible, but has not yet been confirmed, that this antibody recognizes a conformational epitope.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.
Specific Publications For Anti-Cybr CYTIP Antibody (A09710)
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