Anti-MUC1 Rabbit Monoclonal Antibody

Boster Bio Anti-MUC1 Rabbit Monoclonal Antibody catalog # M00187-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00187-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-MUC1 Rabbit Monoclonal Antibody

See all MUC1 products

SKU/Catalog Number

M00187-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MUC1 Rabbit Monoclonal Antibody catalog # M00187-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MUC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00187-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FHA-13

Immunogen

A synthesized peptide derived from human MUC1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00187-1 is reactive to MUC1 in Human

Applications

M00187-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Flow Cytometry 1:20

Validation Images & Assay Conditions

Gene/Protein Information For MUC1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P15941

Gene ID

4582

Gene Name

MUC1

Full Name

Mucin-1

Weight

122.102kDa

Alternative Names

Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 antigen|breast carcinoma-associated antigen DF3|cancer antigen 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MUC1, check out the MUC1 Infographic

MUC1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MUC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00187-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Globose, cystic olfactory ensheathing cell tumor: A case report and literature review

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18 Customer Q&As for Anti-MUC1 Rabbit Monoclonal Antibody

Question

Will M00187-1 anti-MUC1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Patel

Verified customer

Asked: 2020-05-08

Answer

You can see on the product datasheet, M00187-1 anti-MUC1 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-08

Question

Is a blocking peptide available for product anti-MUC1 Rabbit Monoclonal antibody (M00187-1)?

R. Li

Verified customer

Asked: 2020-04-09

Answer

We do provide the blocking peptide for product anti-MUC1 Rabbit Monoclonal antibody (M00187-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-09

Question

I see that the anti-MUC1 Rabbit Monoclonal antibody M00187-1 works with IHC, what is the protocol used to produce the result images on the product page?

R. Johnson

Verified customer

Asked: 2020-03-26

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-26

Question

Do you have a BSA free version of anti-MUC1 Rabbit Monoclonal antibody M00187-1 available?

M. Krishna

Verified customer

Asked: 2020-01-02

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC1 Rabbit Monoclonal antibody M00187-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-02

Question

I have attached the WB image, lot number and protocol we used for carcinoma using anti-MUC1 Rabbit Monoclonal antibody M00187-1. Please let me know if you require anything else.

C. Baker

Verified customer

Asked: 2019-09-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-20

Question

Does anti-MUC1 Rabbit Monoclonal antibody M00187-1 work for IHC with carcinoma?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

According to the expression profile of carcinoma, MUC1 is highly expressed in carcinoma. So, it is likely that anti-MUC1 Rabbit Monoclonal antibody M00187-1 will work for IHC with carcinoma.

Boster Scientific Support

Answered: 2019-07-04

Question

The lowest kDa for this protein in the WB image should be ~100kDa. Could you ask confirm why the kDa for M00187-1 is so low?

Verified customer

Asked: 2019-04-23

Answer

According to Uniprot https://www.uniprot.org/uniprot/P15941, there are 17 isoforms of MUC1. For some of them, the Mass (Da) is around 17-30kDa. Immunogen sequence of the Anti-MUC1 Rabbit Monoclonal Antibody (M00187-1) is CGSSLSYTNPAVAAASANL. The band between 20 and 25kDa is the only one detected by the antibody.

Boster Scientific Support

Answered: 2019-04-23

Question

Our lab used your anti-MUC1 Rabbit Monoclonal antibody for WB on mammary carcinoma in a previous project. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining mammary carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

I have checked the website and datasheets of our anti-MUC1 Rabbit Monoclonal antibody and it appears that M00187-1 has been tested on human in both WB and IHC. Thus M00187-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-06

Question

We are currently using anti-MUC1 Rabbit Monoclonal antibody M00187-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-23

Answer

The anti-MUC1 Rabbit Monoclonal antibody (M00187-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-23

Question

Can you check to be sure the correct WB image is upload for this product? https://www.bosterbio.com/anti-muc1-rabbit-monoclonal-antibody-m00187-1-boster.html?options=cart The size is ~100 kDa low.

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

According to Uniprot https://www.uniprot.org/uniprot/P15941, there are 17 isoforms of MUC1. For some of them, the Mass (Da) are around 17-30kDa. Immunogen sequence of M00187-1 is CGSSLSYTNPAVAAASANL. The band between 20 and 25kDa is the only one detected by them.

Boster Scientific Support

Answered: 2017-12-18

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for carcinoma using anti-MUC1 Rabbit Monoclonal antibody M00187-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-08-09

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-09

Question

I am looking for to test anti-MUC1 Rabbit Monoclonal antibody M00187-1 on human carcinoma for research purposes, then I may be interested in using anti-MUC1 Rabbit Monoclonal antibody M00187-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

The products we sell, including anti-MUC1 Rabbit Monoclonal antibody M00187-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-12

Question

Can you help my question with product M00187-1, anti-MUC1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00187-1 anti-MUC1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-22

Question

I was wanting to use your anti-MUC1 Rabbit Monoclonal antibody for IHC for human carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human carcinoma identification?

J. Parker

Verified customer

Asked: 2016-03-21

Answer

It shows on the product datasheet, M00187-1 anti-MUC1 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-03-21

Question

Our lab were well pleased with the WB result of your anti-MUC1 Rabbit Monoclonal antibody. However we have observed positive staining in lung isoform 9: secreted. using this antibody. Is that expected? Could you tell me where is MUC1 supposed to be expressed?

R. Mangal

Verified customer

Asked: 2015-11-05

Answer

According to literature, lung does express MUC1. Generally MUC1 expresses in apical cell membrane, isoform 5: secreted., isoform y: secreted., isoform 9: secreted., mucin-1 subunit beta: cell membrane. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722

Boster Scientific Support

Answered: 2015-11-05

Question

Does anti-MUC1 Rabbit Monoclonal antibody M00187-1 work on primate IP with carcinoma?

P. Banerjee

Verified customer

Asked: 2014-10-03

Answer

Our lab technicians have not validated anti-MUC1 Rabbit Monoclonal antibody M00187-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MUC1 Rabbit Monoclonal antibody M00187-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate carcinoma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-03

Question

We have observed staining in human body of stomach. Are there any suggestions? Is anti-MUC1 Rabbit Monoclonal antibody supposed to stain body of stomach positively?

C. Baker

Verified customer

Asked: 2013-08-08

Answer

From what I have seen in literature body of stomach does express MUC1. From what I have seen in Uniprot.org, MUC1 is expressed in body of stomach, pancreatic carcinoma, mammary carcinoma, carcinoma, cervix carcinoma, thyroid, lung, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722

Boster Scientific Support

Answered: 2013-08-08

Question

Is this M00187-1 anti-MUC1 Rabbit Monoclonal antibody reactive to the isotypes of MUC1?

T. Johnson

Verified customer

Asked: 2013-02-14

Answer

The immunogen of M00187-1 anti-MUC1 Rabbit Monoclonal antibody is A synthesized peptide derived from human MUC1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-02-14

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M00187-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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