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Product Info Summary
| SKU: | M00211 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Myelin Basic Protein MBP Rabbit Monoclonal Antibody
SKU/Catalog Number
M00211
BM4869 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myelin Basic Protein MBP Rabbit Monoclonal Antibody catalog # M00211. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin Basic Protein MBP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00211)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IIE-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myelin Basic Protein
Reactive Species
M00211 is reactive to MBP in Human
Observed Molecular Weight
18 kDa
Calculated molecular weight
33.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00211 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
IHC: human brain tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human brain, using Myelin Basic Protein Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Myelin Basic Protein expression in Human fetal brain lysate.
Specific Publications For Anti-Myelin Basic Protein MBP Rabbit Monoclonal Antibody (M00211)
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Customer Q&As
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3 Customer Q&As for Anti-Myelin Basic Protein MBP Rabbit Monoclonal Antibody
Question
My team were satisfied with the WB result of your anti-Myelin Basic Protein Rabbit Monoclonal antibody. However we have been able to see positive staining in brain myelin membrane using this antibody. Is that expected? Could you tell me where is MBP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Based on literature, brain does express MBP. Generally MBP expresses in myelin membrane. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-Myelin Basic Protein Rabbit Monoclonal antibody M00211 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
The anti-Myelin Basic Protein Rabbit Monoclonal antibody (M00211) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-12
Question
We have seen staining in human leukemic t-cell. What should we do? Is anti-Myelin Basic Protein Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
From what I have seen in literature leukemic t-cell does express MBP. From what I have seen in Uniprot.org, MBP is expressed in c1 segment of cervical spinal cord, embryonic spinal cord, brain, amygdala, hippocampus subthalamic nucleus, brain, lung skin, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2017-05-12
