Anti-Oct4/POU5F1 Antibody

POU5F1 antibody

Boster Bio Anti-Oct4/POU5F1 Antibody catalog # A00174-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00174-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

Product Name

Anti-Oct4/POU5F1 Antibody

View all POU5F1 Antibodies

SKU/Catalog Number

A00174-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Oct4/POU5F1 Antibody catalog # A00174-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Oct4/POU5F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Oct4/POU5F1 recombinant protein (Position: E26-A300).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00174-1 is reactive to POU5F1 in Human, Mouse, Rat

Applications

A00174-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Background of POU5F1

Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For POU5F1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q01860

Gene ID

5460

Gene Name

POU5F1

Full Name

POU domain, class 5, transcription factor 1

Weight

38.571kDa

Superfamily

POU transcription factor family

Alternative Names

class 5, transcription factor 1; MGC22487; Oct-3; OCT3Oct4; Oct-4; Octamer-binding protein 3; otc-4; OTF3POU domain class 5, transcription factor 1; OTF4; POU class 5 homeobox 1; POU-type homeodomain-containing DNA-binding protein POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 POU domain, class 5, transcription factor 1|POU domain transcription factor OCT4|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on POU5F1, check out the POU5F1 Infographic

POU5F1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for POU5F1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00174-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis

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1 Customer Q&As for Anti-Oct4/POU5F1 Antibody

Question

We are currently using anti-Oct4/POU5F1 antibody A00174-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-05

Answer

The anti-Oct4/POU5F1 antibody (A00174-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-05

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00174-1

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A00174-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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