Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody

Product Name

Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody

View all Sodium Potassium ATPase Alpha 1 Antibodies

SKU/Catalog Number

A00956Y10

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody catalog # A00956Y10. Tested in WB,IHC,IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00956Y10)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Na+/K+-ATPase alpha1 around the phosphorylation site of S16.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00956Y10 is reactive to ATP1A1 in Human

Applications

A00956Y10 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

112.896kDa

Background of Sodium Potassium ATPase Alpha 1

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.

Fernandez-Salguero P., Am. J. Hum. Genet. 57:651-660(1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of ATP1A1 using Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody (A00956Y10).

Immunohistochemistry (IHC) analyzes of Na+/K+-ATPase α1 (Y10) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody A00956Y10

Western blot (WB) analysis of Na+/K+-ATPase α1 (Y10) pAb at 1:500 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ATP1A1 (Source: Uniprot.org, NCBI)

Alternative Names

ATPase, Na+/K+ transporting, alpha 1 polypeptide; EC 3.6.3; EC 3.6.3.9; K-ATPase alpha-1 subunit; K-ATPase catalytic subunit alpha-A protein; MGC3285; MGC51750; Na; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide ATP1A1 CMT2DD, HOMGSMR2 ATPase Na+/K+ transporting subunit alpha 1 sodium/potassium-transporting ATPase subunit alpha-1|ATPase, Na+/K+ transporting, alpha 1 polypeptide|Na(+)/K(+) ATPase alpha-1 subunit|Na+/K+ ATPase 1|Na, K-ATPase, alpha-A catalytic polypeptide|Na,K-ATPase alpha-1 subunit|Na,K-ATPase catalytic subunit alpha-A protein|sodium pump subunit alpha-1|sodium-potassium ATPase catalytic subunit alpha-1|sodium-potassium-ATPase, alpha 1 polypeptide

For more info on ATP1A1, check out the ATP1A1 Infographic

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In this infographic, you will see the following information for ATP1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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