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Product Info Summary
|Reactive Species:||Human, Monkey|
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Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband™
Boster Bio Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband™ catalog # PB9482. Tested in WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9482)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1 (7-34aa RDKYEPAAVSEQGDKKGKKGKKDRDMDE), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9482 is reactive to ATP1A1 in Human, Monkey
PB9482 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Alpha 1 Sodium Potassium ATPase/ATP1A1 using anti-Alpha 1 Sodium Potassium ATPase/ATP1A1 antibody (PB9482).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human SW620 whole cell lysates,
Lane 7: monkey COS-7 whole cell lysates,
Lane 8: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Sodium Potassium ATPase/ATP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9482) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Sodium Potassium ATPase/ATP1A1 at approximately 100-113KD. The expected band size for Alpha 1 Sodium Potassium ATPase/ATP1A1 is at 113KD.
Gene/Protein Information For ATP1A1 (Source: Uniprot.Org, NCBI)
Sodium/potassium-transporting ATPase subunit alpha-1
cation transport ATPase (P-type) (TC 3.A.3) family
ATPase, Na+/K+ transporting, alpha 1 polypeptide; EC 3.6.3; EC 126.96.36.199; K-ATPase alpha-1 subunit; K-ATPase catalytic subunit alpha-A protein; MGC3285; MGC51750; Na; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide ATP1A1 CMT2DD, HOMGSMR2 ATPase Na+/K+ transporting subunit alpha 1 sodium/potassium-transporting ATPase subunit alpha-1|ATPase, Na+/K+ transporting, alpha 1 polypeptide|Na(+)/K(+) ATPase alpha-1 subunit|Na+/K+ ATPase 1|Na, K-ATPase, alpha-A catalytic polypeptide|Na,K-ATPase alpha-1 subunit|Na,K-ATPase catalytic subunit alpha-A protein|sodium pump subunit alpha-1|sodium-potassium ATPase catalytic subunit alpha-1|sodium-potassium-ATPase, alpha 1 polypeptide*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ATP1A1, check out the ATP1A1 Infographic
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In this infographic you will see the following information for ATP1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9482
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