ATP1A1 Antibodies

AND ATP1A1 ELISA kits, ATP1A1 Proteins

Many biological assays use antibodies to detect ATP1A1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ATP1A1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ATP1A1 antibodies...

We validate the specificity of these antibodies to ATP1A1 by testing them on tissues known to express ATP1A1 positively and negatively. Browse below to find the ATP1A1 antibody that suites your experiment. We have 14 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for ATP1A1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the ATP1A1 Western Blot Guide

ATP1A1 Infographic by Boster Bio

All ATP1A1 Products

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Applications

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Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband®

4 Citations

Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482)

IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482)

Human, Mouse, Rat

IF, IHC, WB

$370
Cat # PB9482
100 μg/vial

Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody

1 Citations

All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M00956
100 μl

Anti-Na+/K+-ATPase alpha1 Antibody

3 Citations

Immunofluorescence analysis of COS7 cells, using ATPase Antibody

Western Blot analysis of 293 cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells, treated with PMA 125ng/ml 30

Human, Mouse, Rat

ELISA, IF, IHC, WB

$399
Cat # A00956
100μl

Anti-Na+/K+-ATPase Alpha 1 (Y10) ATP1A1 Antibody

Western blot (WB) analysis of Na+/K+-ATPase α1 (Y10) pAb at 1:500 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)

Human

IF, IHC, WB

$405
Cat # A00956Y10
100ul

Anti-Na+/K+-ATPase Alpha 1 (phospho-S16) ATP1A1 Antibody

Western blot (WB) analysis of p-Na+/K+-ATPase α1 (S16) pAb at 1:2000 dilution
Lane1:HEK293T whole cell lysate
Lane2:HEK293T treated with EGF(100ng/ml,15 minutes) whole cell lysate
Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate
Lane4:C6 whole cell lysate
Lane5:NIH-3T3 whole cell lysate

Immunohistochemistry (IHC) analyzes of p-Na+/K+-ATPase α1 (S16) pAb in paraffin-embedded human breast carcinoma tissue at 1:50

Human, Mouse, Rat

IHC, WB

$482
Cat # A00956S16
100ul

Anti-Sodium Potassium ATPase alpha-1 ATP1A1 Antibody (Phospho-Tyr260)

Western blot analysis of KB Hela lysate, antibody was diluted at 1000

Western blot analysis of lysates from 293 cells treated with PMA, using phospho-Na+/K+-ATPase α1 (Phospho-Tyr260) antibody

Immunohistochemical analysis of paraffin-embedded human tonsil

Human, Mouse, Rat

ELISA, IHC, WB

$380
Cat # A00956Y260
100ul

ATP1A1 (Phospho-Ser23) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from rat brain

Rat

ELISA

$540
Cat # EKC2314
1 kit, containing two 96-well plates and all necessary reagents

ATP1A1 (Phospho-Ser16) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30

Human, Mouse, Rat

ELISA

$540
Cat # EKC2054
1 kit, containing two 96-well plates and all necessary reagents

ATP1A1 Colorimetric Cell-Based ELISA Kit

Western blot analyzes of Na+/K+-ATPase alpha1 antibody in extracts from A549 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1045
1 kit, containing one 96-well plate and all necessary reagents

Anti-Na+/K+-ATPase ATP1A1 Antibody

Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution

ICC staining Sodium Potassium ATPase in Hela cells (green)

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$405
Cat # A00956-3
100ul

ATPase Colorimetric Cell-Based ELISA

Western blot analysis of extracts from 293 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1928
1 kit, containing one 96-well plate and all necessary reagents

Sodium Potassium ATPase (ATP1A1) (NM_000701) Human Over-expression Lysate

Sodium Potassium ATPase (ATP1A1) Human Over-expression Lysates NM_000701

Human

$628
Cat # LS000476
20 µg, 100 µg

Anti-ATP1A1 (phospho-S23) Antibody

Western blot (WB) analysis of p-ATP1A1(S23) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:DLD whole cell lysate(40ug)

Human

$482
Cat # A00956S23
100ul

Anti-Phospho-Na+/K+-ATPase alpha (S23) ATP1A1 Antibody

Rat

ELISA, IF, WB

$380
Cat # A00956S23-1
100ul

ATP1A1 Overview

Facts about Sodium/potassium-transporting ATPase subunit alpha-1.

ATP1A1 3D structure. Source: alphafold

Sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1)

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Gene Information

Human
Gene Name:	ATP1A1
Uniprot:	P05023
Entrez:	476

Family

Belongs to:

cation transport ATPase (P-type) (TC 3.A.3) family

Alternative Names

ATPase, Na+/K+ transporting, alpha 1 polypeptide; EC 3.6.3; EC 3.6.3.9; K-ATPase alpha-1 subunit; K-ATPase catalytic subunit alpha-A protein; MGC3285; MGC51750; Na; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide

Molecular Weight

Mass (kDA):
112.896 kDA

Genomic Context


Human

Location:	1p13.1
Sequence:	1; NC_000001.11 (116372986..116410259)

Subcellular Localizations

Cell membrane, sarcolemma; Multi-pass membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Diseases

Hypertensive Disease
Retinoblastoma
Migraine Disorders
Malignant Neoplasms
Neoplasms
Diabetes Mellitus
Migraine With Aura
Heart Failure
Ischemia
Diabetes Mellitus, Non-insulin-dependent
Familial Hemiplegic Migraine
Disease Of Adrenal Medulla

Endometriosis, Site Unspecified
Essential Hypertension
Hypertrophy
Tissue Adhesions
Headache
Reperfusion Injury
Bipolar Disorder

Interacting Proteins

ATP1B1
TPT1

Pathways

Transport
Localization
Excretion
Secretion
Pathogenesis
Methylation
Ion Transport
Endocytosis
Cell Proliferation
Acid Secretion
Regulation Of Blood Pressure
Glomerular Filtration
Cell Death

Transepithelial Transport
Sperm Motility
Acrosome Reaction
Fertilization
Blastocyst Formation
Membrane Depolarization
Blastocyst Development

PTMs

Phosphorylation
Dephosphorylation
Methylation

Cleavage
Biotinylation
Glutathionylation

Deglutathionylation

Research Areas

Cancer
Cellular Markers
Growth and Development
Hypoxia
Neuronal Cell Markers

Neuroscience
Plasma Membrane Markers
Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATP1A1

References

PMID: 2430951 by Kawakami K., et al. Primary structure of the alpha-subunit of human Na,K-ATPase deduced from cDNA sequence.

PMID: 7536695 by Ruiz A., et al. Characterization and quantification of full-length and truncated Na,K-ATPase alpha 1 and beta 1 RNA transcripts expressed in human retinal pigment epithelium.

1 Publications of Boster ATP1A1 Antibodies & ELISA Kits

PubMed ID: 32922293, Li H,Ma Z,Zhai Y,Lv C,Yuan P,Zhu F,Wei L,Li Q,Qi X.Trimetazidine Ameliorates Myo...

*More publications can be found for each product on its corresponding product page