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Product Info Summary
| SKU: | M03589-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Natriuretic Peptide Receptor C (NPR3) Mouse Monoclonal Antibody [Clone ID: OTI11B6]
SKU/Catalog Number
M03589-3
Size
100 µl
Description
Boster Bio Anti-NPR3 (Natriuretic Peptide Receptor C) mouse monoclonal antibody, clone OTI11B6 (formerly 11B6). Catalog# M03589-3. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Natriuretic Peptide Receptor C (NPR3) Mouse Monoclonal Antibody [Clone ID: OTI11B6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03589-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI11B6
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.
Reactive Species
M03589-3 is reactive to NPR3 in Human, Mouse, Rat
Calculated molecular weight
59.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03589-3 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200 - 1:1000
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPR3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPR3.
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Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NPR3 monoclonal antibody at 1:200.
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either pCMV6-ENTRY NPR3 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NPR3 mouse monoclonal (M03589-3)
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Anti-NPR3 mouse monoclonal antibody (M03589-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NPR3.
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Immunofluorescent staining of HeLa cells using anti-NPR3 mouse monoclonal antibody (M03589-3).
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Immunofluorescent staining of HT29 cells using anti-NPR3 mouse monoclonal antibody (M03589-3).
Specific Publications For Anti-Natriuretic Peptide Receptor C (NPR3) Mouse Monoclonal Antibody [Clone ID: OTI11B6] (M03589-3)
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