|Product Name||Anti-DUT Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial(DUT) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-DUT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1030-1)|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human DUT(212-229aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-DUT antibody, PA1030-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-DUT antibody, PA1030-1, Western blotting
All lanes: Anti DUT (PA1030-1) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial|
|Tissue Specificity||Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). .|
|Alternative Names||Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial;dUTPase;188.8.131.52;dUTP pyrophosphatase;DUT;|
|Subcellular Localization||Isoform 2: Nucleus.|
|Molecular Weight||26563 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. .|
|Background||Deoxyuridine triphosphate nucleotidohydrolase(dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E.coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,