Anti-NEMO/IKK-gamma IKBKG Antibody

Product Name

Anti-NEMO/IKK-gamma IKBKG Antibody

View all IKBKG Antibodies

SKU/Catalog Number

A00874-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NEMO/IKK-gamma IKBKG Antibody (Catalog # A00874-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-NEMO/IKK-gamma IKBKG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00874-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00874-1 is reactive to IKBKG in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

48.2 kDa

Background of IKBKG

Anti-NEMO antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-NEMO antibody detects recombinant and endogenous NEMO. NEMO, the regulatory subunit of the IKK core complex, phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Nemo is also considered to be a mediator for TAX activation of NF-kappa-B and may be implicated in NF-kappa-B-mediated protection from cytokine toxicity. NEMO is essential for viral activation of IRF3 and involved in TLR3- and IFIH1-mediated antiviral innate response. The innate antiviral response from NEMO requires 'Lys-27'-linked polyubiquitination. Anti-NEMO is ideal for researchers interested in Immunology and Cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00874-1 is guaranteed for ELISA, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:50,000 - 1:400,000
IP: 5µg
WB: 1:500-1:1000
Anti-NEMO antibody has been tested by western blot and is suitable for immunoprecipitation and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48kDa in size corresponding to endogenous NEMO protein by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information IKBKG

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