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NTN4 antibody (A07052-1) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of SIHA cells using anti-NTN4 antibody (A07052-1).
Overlay histogram showing SIHA cells stained with A07052-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NTN4 Antibody (A07052-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SIHA cells stained with A07052-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NTN4 Antibody (A07052-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NTN4 Antibody Picoband®
NTN4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
NTN4 antibody was positively detected by WB in:
human RT4 whole cell, human A549 whole cell, human PC-3 whole cell, human SIHA whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NTN4 antibody was positively detected by IHC in:
human endometrial cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NTN4 antibody was positively detected by FC in:
SIHA cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NTN4 Antibody Picoband®
View all NTN4 Antibodies
View all NTN4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NTN4 recombinant protein (Position: C端). Human NTN4 shares 81% amino acid (aa) sequence identity with mouse NTN4.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
70.1 kDa
Background of NTN4
This gene encodes a member of the netrin family of proteins, which function in various biological processes including axon guidance, tumorogenesis, and angiogenesis. Netrins are laminin-related proteins that have an N-terminal laminin-type domain, epidermal growth factor-like repeat domain, and a positively charged heparin-binding domain at the C-terminus. The protein encoded by this gene is involved in processes including neurite growth and migration, angiogenesis and mural cell adhesion to endothelial cells. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-NTN4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07052-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Cow
95%
High Homology
Monkey
95%
High Homology
Mouse
77%
Moderate Homology
Rat
77%
Moderate Homology
Zebrafish
45%
Low homology
Drosophila
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NTN4 antibody
Immunohistochemistry (IHC) protocol for NTN4 antibody
Flow Cytometry (FC) protocol for NTN4 antibody
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Product Promise
We promise Anti-NTN4 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A07052-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026