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ENO2 antibody (A02930) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 25 Publications

a02930 nse primary antibodies fcm testing 8

Flow Cytometry analysis of A431 cells using anti-NSE antibody (A02930).
Overlay histogram showing A431 cells stained with A02930 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NSE Antibody (A02930, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-NSE/ENO2 Antibody Picoband®

ENO2 antibody

25 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

ENO2 antibody was positively detected by WB in:

human Jurkat whole cell, human SH-SY5Y whole cell, human U2OS whole cell, human K562 whole cell, rat brain tissue, rat skeletal muscle tissue, mouse brain tissue, mouse skeletal muscle tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ENO2 antibody was positively detected by IHC in:

human lung cancer tissue, human placenta tissue, human pancreatic cancer tissue, rat brain tissue, mouse brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ENO2 antibody was positively detected by ICC/IF in:

A431 cell

ENO2 antibody was positively detected by FC in:

A431 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Dog

Predicted

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-NSE/ENO2 Antibody Picoband®
View all ENO2 Antibodies

Form

Lyophilized

Uniprot

P09104

Gene ID

2026

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NSE, which shares 95.1% and 100% amino acid (aa) sequence identity with mouse and rat NSE, respectively.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

47 kDa

Calculated Molecular Weight

47.3 kDa

Background of ENO2

NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Cite This Product

Anti-NSE/ENO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02930)

Protocols

Western Blot protocol for ENO2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ENO2 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ENO2 antibody

Review Protocol

Flow Cytometry (FC) protocol for ENO2 antibody

Review Protocol

Publications

Elevated Release of Presynaptic Glutamate: The Potential Pathogenesis of Anti-NMDAR Encephalitis-Associated Seizures

Published: 2025-10-17

Journal: CNS Neuroscience & Therapeutics

Application: IF

Impact Factor: 5.00

Species: Rat

PMID: 41104530

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Free Secondary Antibody

Anti-NSE/ENO2 Antibody Picoband®

ENO2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Elevated Release of Presynaptic Glutamate: The Potential Pathogenesis of Anti-NMDAR Encephalitis-Associated Seizures

Lactate-Dehydrogenase-5 May Play a Key Role in the Disturbance of Brain Energy Caused by Tuberculous Meningitis

Renal and cerebral RAS interaction contributes to diabetic kidney disease

The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury

Biofunctionalized self-assembly of peptide amphiphile induces the differentiation of bone marrow mesenchymal stem cells into neural cells

Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling

Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene

Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury

Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells

Neuroprotective effects of oxysophocarpine on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion

β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells

Mdivi-1 Prevents Apoptosis Induced by Ischemia–Reperfusion Injury in Primary Hippocampal Cells via Inhibition of Reactive Oxygen Species–Activated Mitochondrial Pathway

Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia

Amyloid Beta-Derived Diffusible Ligands (ADDLs) Induce Abnormal Expression of Insulin Receptors in Rat Hippocampal Neurons

Wnt1 from cochlear schwann cells enhances neuronal differentiation of transplanted neural stem cells in a rat spiral ganglion neuron degeneration model.

Injury of cortical neurons is caused by the advanced glycation end products-mediated pathway

Expression pattern of sorting nexin 25 in temporal lobe epilepsy: A study on patients and pilocarpine-induced rats

Brain targeting and toxicity study of odorranalectin-conjugated nanoparticles following intranasal administration

Modulation of Migration and Ca2+ Signaling in Retinal Pigment Epithelium Cells by Recombinant Human CTGF

The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice

The structural characteristics and expression of neuropeptides in the esophagus of patients with congenital esophageal atresia and tracheoesophageal fistula

UEA I-bearing nanoparticles for brain delivery following intranasal administration

Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells

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