Anti-NF-Y NFYB Antibody

Product Name

Anti-NF-Y NFYB Antibody

View all NFYB Antibodies

SKU/Catalog Number

A06714-1

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NF-Y NFYB Antibody (Catalog # A06714-1). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-NF-Y NFYB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06714-1)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactive Species

A06714-1 is reactive to NFYB in Human

Observed Molecular Weight

39 kDa

Calculated molecular weight

22.8 kDa

Background of NFYB

Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06714-1 is guaranteed for ELISA, IHC, WB, EMSA Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
EMSA: 0.5 to 1.0 µL
IHC: 1:500
WB: 1:500 - 1:3,000
This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y.  Minimal reactivity was observed against the A subunit of NF-Y.  This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 µl per assay. This product is tested for immunohistochemistry in human thymus tissue at 1:500.

Validation Images & Assay Conditions

See full target information NFYB

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