Anti-MNAT1 Antibody Picoband™

MNAT1 antibody

Boster Bio Anti-MNAT1 Antibody Picoband™ catalog # PB9614. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9614
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-MNAT1 Antibody Picoband™

View all MNAT1 Antibodies

SKU/Catalog Number

PB9614

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MNAT1 Antibody Picoband™ catalog # PB9614. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MNAT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9614)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MNAT1 recombinant protein (Position: L92-A278). Human MNAT1 shares 93% amino acid (aa) sequence identity with mouse MNAT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9614 is reactive to MNAT1 in Human, Mouse, Rat

Applications

PB9614 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

35.823kDa

Background of MNAT1

CDK-activating kinase assembly factor MAT1 is an enzyme that in humans is encoded by the MNAT1 gene. The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MNAT1 (Source: Uniprot.org, NCBI)

Gene Name

MNAT1

Full Name

CDK-activating kinase assembly factor MAT1

Weight

35.823kDa

Alternative Names

CAP35; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1TFB3; menage a trois 1 (CAK assembly factor); menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis); Menage a trois; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66CDK-activating kinase assembly factor MAT1 MNAT1 CAP35, MAT1, RNF66, TFB3 MNAT1 component of CDK activating kinase CDK-activating kinase assembly factor MAT1|CDK7/cyclin-H assembly factor|MNAT CDK-activating kinase assembly factor 1|MNAT1, CDK activating kinase assembly factor|RING finger protein 66|RING finger protein MAT1|cyclin G1 interacting protein|menage a trois 1 (CAK assembly factor)|menage a trois homolog 1, cyclin H assembly factor|menage a trois-like protein 1 cyclin H assembly factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MNAT1, check out the MNAT1 Infographic

MNAT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MNAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-MNAT1 Antibody Picoband™

Question

Can you help my question with product PB9614, anti-MNAT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9614 anti-MNAT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-20

Question

I have attached the WB image, lot number and protocol we used for liver using anti-MNAT1 antibody PB9614. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-08

Question

We are currently using anti-MNAT1 antibody PB9614 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

The anti-MNAT1 antibody (PB9614) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-01

Question

Would anti-MNAT1 antibody PB9614 work for IHC with liver?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

According to the expression profile of liver, MNAT1 is highly expressed in liver. So, it is likely that anti-MNAT1 antibody PB9614 will work for IHC with liver.

Boster Scientific Support

Answered: 2019-06-11

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-MNAT1 antibody PB9614. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-10-04

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-04

Question

My question regards to test anti-MNAT1 antibody PB9614 on human liver for research purposes, then I may be interested in using anti-MNAT1 antibody PB9614 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Johnson

Verified customer

Asked: 2016-03-03

Answer

The products we sell, including anti-MNAT1 antibody PB9614, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-03-03

Question

I was wanting to use your anti-MNAT1 antibody for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

H. Banerjee

Verified customer

Asked: 2015-10-27

Answer

It shows on the product datasheet, PB9614 anti-MNAT1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-10-27

Order DetailsPrice
PB9614

100μg

$370
PB9614-10ug

10μg sample (liquid)

$99
PB9614-Biotin

100 μg Biotin conjugated

$570
PB9614-Cy3

100 μg Cy3 conjugated

$570
PB9614-Dylight488

100 μg Dylight488 conjugated

$570
PB9614-Dylight550

100 μg Dylight550 conjugated

$570
PB9614-Dylight594

100 μg Dylight594 conjugated

$570
PB9614-FITC

100 μg FITC conjugated

$570
PB9614-HRP

100 μg HRP conjugated

$570
PB9614-APC

100 μg APC conjugated

$670
PB9614-PE

100 μg PE conjugated

$670
PB9614-carrier-free

Carrier Free

$370

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PB9614
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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