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Product Info Summary
| SKU: | M03515-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-NONO Mouse Monoclonal Antibody [Clone ID: OTI4D9]
SKU/Catalog Number
M03515-1
Size
100 µl
Description
Boster Bio NONO mouse monoclonal antibody, clone OTI4D9 (formerly 4D9). Catalog# M03515-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NONO Mouse Monoclonal Antibody [Clone ID: OTI4D9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03515-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4D9
Isotype
IgG1
Immunogen
Human recombinant protein fragment correspongding to amino acids 184-385 of human NONO (NP_031389) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03515-1 is reactive to NONO in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
54.232kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03515-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either NONO (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody (M03515-1)
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Anti-NONO mouse monoclonal antibody (M03515-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NONO monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and NONO-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-NONO monoclonal antibody M03515-1 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-NONO monoclonal antibody (1:200).
Protein Target Info & Infographic
Gene/Protein Information For NONO (Source: Uniprot.org, NCBI)
Gene Name
NONO
Full Name
Non-POU domain-containing octamer-binding protein
Weight
54.232kDa
Alternative Names
NMT5552 kDa subunit; non-POU domain containing, octamer-binding; NRB54non-POU-domain-containing, octamer-binding; p54(nrb) NONO MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114 non-POU domain containing octamer binding non-POU domain-containing octamer-binding protein|54 kDa nuclear RNA- and DNA-binding protein|55 kDa nuclear protein|DNA-binding p52/p100 complex, 52 kDa subunit|non-POU domain-containing octamer (ATGCAAAT) binding protein|p54(nrb)|protein phosphatase 1, regulatory subunit 114
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NONO, check out the NONO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NONO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NONO Mouse Monoclonal Antibody [Clone ID: OTI4D9] (M03515-1)
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