Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband®

Anti-TGF beta Receptor II antibody, PA1866, Western blotting
All lanes: Anti TGF beta Receptor II (PA1866) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD

Hsa_circ_0009096 sponges miR-370-3p to modulate TGFBR2 expression in LX-2 cells. (A) LX-2 cells were transfected with hsa_circ_0009096 siRNAs for 24 h and then RT-qPCR was used to assess the interference effect of siRNAs ( n = 3 biological replicates per group). (B) Relative mRNA expression of potential target genes of hsa_circ_0009096 ( SMAD4, TGFB2, TGFBR2, and THBS1 ) in LX-2 cells after transfection with hsa_circ_ 0009096 siRNA3 for 24 h were detected via RT-qPCR ( n = 3 biological replicates per group). (C) The expression of miR-21-5p and miR-370-3p in LX-2 cells transfected with hsa_circ_ 0009096 siRNA3 for 24 h were analyzed by the RT-qPCR ( n = 3 biological replicates per group). (D) The interaction between hsa_circ_0009096 and miR-370-3p in 293T cells was confirmed by the dual luciferase reporter assay ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; SMAD4: Mothers Against Decepentaplegic Homolog 4; TGFB2: Transforming Growth Factor Beta 2; THBS1: Thrombospondin 1; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 38766485

Hsa_circ_0009096 silencing suppressed TGF-β1-induced HSC proliferation and fibrosis. LX-2 cells were transfected with siNC or hsa_circ_0009096 siRNA3 for 24 h, followed by treatment without or with 2 ng/mL rhTGF-β1 for 24 h. (A–D) The miRNA/mRNA expression of a-SMA, COL1A1, TGFBR2, and miR-370-3p were detected by RT-qPCR ( n = 3 biological replicates per group). (E–F) The cell cycle progression and the apoptosis of LX-2 cells were assessed by flow cytometry ( n = 3 biological replicates per group). (G) Alterations of a-SMA and COL1A1 protein abundances in LX-2 cells were evaluated by Immunocytochemistry ( n = 3 biological replicates per group). (H) The protein expression of TGFBR2, COL1A1, and a-SMA was examined by western blotting ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; COL1A1: collagen 1A1; α-SMA: alpha-smooth muscle actin; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 38766485

MiR-370-3p mediated hsa_circ_0009096-regulated proliferation and fibrosis of TGF-β1-treated HSCs. LX-2 cells were transfected with siNC+NC-inhibitor, hsa_circ_0009096 siRNA3+NC-inhibitor, and hsa_circ_0009096 siRNA3+miR-370-3p-inhibitor, respectively, for 24 h, followed by treatment with 2 ng/mL rhTGF-β1 for 24 h. (A–D) The miRNA/mRNA expression of miR-370-3p, TGFBR2, COL1A1, and a-SMA were detected by RT-qPCR ( n = 3 biological replicates per group). (E–F) The cell cycle progression and the apoptosis of LX-2 cells were assessed by flow cytometry ( n = 3 biological replicates per group). (G) Alterations of a-SMA and COL1A1 protein abundances in LX-2 cells were evaluated by Immunocytochemistry ( n = 3 biological replicates per group). (H) The protein expression of TGFBR2, COL1A1, and a-SMA was examined by western blotting ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; COL1A1: collagen 1A1; α-SMA: alpha-smooth muscle actin; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 38766485

Bioinformatics analysis of DEPs. All 522 DEPs underwent the analysis of subcellular localization ( A ), the enrichment analysis of KEGG signaling pathways ( B ) and Gene Ontology annotation ( C ). ( D ) The DEPs in the “Proteoglycans in cancer” KEGG pathway. ( E ) Western blot analysis of TGF-β2, SMAD2, and p-SMAD2 in PANC-1 cells treated with or without rhoifolin. GAPDH was used as internal reference.
Index in PubMed under a CC BY license. PMID: 35383226